UV-Induced Spectral Analysis for Rapid Liquid Sample Identification
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Solution Overview
Problem
Current analytical methods for identifying properties of diverse pharmacological, food, and biological samples are time-consuming and costly, requiring complex sample preparation and instrumentation, and often struggle with precise quantitative and qualitative analysis, especially in emergency situations or for rapid drug determination.
Innovation Solution
A method utilizing UV radiation at 250-400 nm to induce spectral changes in samples, allowing for rapid identification of properties through fluorescence spectroscopy without extensive sample preparation, using metal ions like Zn2+, Cu2+, and disulfide bonds, which forms quantum nanocrystals, radicals, or degrades chemical bonds, enabling quick analysis with low instrument demands.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If extraction, isolation and separation processes are performed prior to analysis, then measurement precision is improved, but loss of time increases and device complexity increases
Solution Approach 1:
The invention extracts only the essential spectral information directly from the sample without performing full extraction, isolation and separation processes. By using UV irradiation to induce spectral changes and analyzing these changes directly, the method removes the time-consuming preparation steps while retaining the ability to identify substance properties through characteristic spectral patterns.
Solution Approach 2:
The method applies UV irradiation as a preliminary action to induce spectral changes in the sample before analysis. This pre-treatment step creates detectable spectral patterns that directly reveal substance properties, eliminating the need for subsequent extraction and separation steps while maintaining analytical precision.
2Measurement precision
If extraction, isolation and separation processes are performed prior to analysis, then measurement precision is improved, but device complexity increases
Solution Approach 1:
The invention extracts only the essential spectral information directly from the sample without performing full extraction, isolation and separation processes. By using UV irradiation to induce spectral changes and analyzing these changes directly, the method removes the time-consuming preparation steps while retaining the ability to identify substance properties through characteristic spectral patterns.
Solution Approach 2:
The invention replaces complex mechanical separation and isolation systems with a UV irradiation and spectral analysis system. Instead of using multiple separation columns, centrifuges, and filtration devices, the method uses electromagnetic radiation to induce spectral changes that directly reveal substance composition, significantly simplifying the instrumentation required.
3Ease of operation
If Raman spectroscopy is used to identify analytes, then ease of operation is improved, but measurement precision deteriorates due to low intensity and fluorescence impurities
Solution Approach 1:
The invention changes the excitation parameter from visible laser light (Raman) to UV radiation. This parameter change induces different spectral responses in the sample, creating stronger and more distinctive spectral patterns that are less susceptible to fluorescence interference. The UV-induced spectral changes provide enhanced detection sensitivity while maintaining operational simplicity.
Solution Approach 2:
The invention converts the potential harm of UV radiation (which could cause photochemical reactions) into a beneficial effect. By using UV irradiation to induce specific spectral changes and analyzing these changes, the method transforms what could be a source of interference into a powerful tool for enhancing detection sensitivity and distinguishing substance properties.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid analysis of samples within minutes, reducing costs and complexity, allowing for precise identification of substances and their properties, suitable for various industries and applications, including drug analysis, food quality control, and disease diagnosis.
Implementation Method 1
The spectra obtained are the result of UV radiation caused by the photooxidation of thiol groups (SH) and subsequent interaction with metal ions
Implementation Method 2
Furthermore, free radicals are generated under the influence of UV radiation, which interact with other molecules
Implementation Method 3
A method for identifying the properties of a sample, in particular a liquid sample... uses natural spectral properties of substances, i.e. the absorption and emission of radiation
Data Source
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AI summary
The present invention relates to a method for identifying properties of substances, in particular liquid substances, wherein the method is based on subjecting a sample (5) of a substance containing on the one hand metal ions and on the other hand a thiol group or disulfide bonds first to the analysis in the original or untreated state, i.e. in the state not irradiated by UV radiation (2, 3, 4), and subsequently subjecting the sample to UV radiation (2, 3, 4), thereby causing spectral changes (10) which are characteristic for the given substance, and investigating the optical properties and possible differences between the original substance and the examined sample of substances based on the change in the spectral characteristics of the sample prior to its irradiation or information stored in the database about the non-radiated sample, and after its irradiation with UV radiation (2, 3, 4). Preferably, the UV radiation (2, 3, 4) in the sample (5) generates quantum nanocrystals (6), free radicals (7), degrades chemical bonds (8) or photo-bleaches (9).