vEPO Protein Variant Enhances Stem Cell Viability and Differentiation

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Solution Overview

Problem

Current methods for expanding and differentiating stem or progenitor cells face challenges such as low viability, limited differentiation potential, and immune rejection, making it difficult to effectively use these cells for therapeutic treatments, particularly in regenerative medicine.

Innovation Solution

The use of a vEPO protein variant in cell culture media to enhance the proliferation, survival, and differentiation of stem and progenitor cells, including neural, hematopoietic, and mesenchymal stem cells, by promoting a supportive environment for their expansion and function.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If stem or progenitor cells are expanded in culture for therapeutic treatments, then the number of cells increases, but cell viability decreases and differentiation potential is limited

Engineering Contradiction:

Improvenumber of stem or progenitor cellsVSAvoidcell viability and differentiation potential

Core Design Contradiction:

Quantity of substanceVSReliability

Solution Approach 1:

The patent applies parameter changes by modifying the chemical composition parameters of the culture medium, specifically incorporating vEPO protein variants and other growth factors to optimize cell expansion while maintaining viability and differentiation potential

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent uses vEPO protein variants as intermediary substances that mediate between the culture medium and stem cells, enhancing cell survival, proliferation, and differentiation through binding to EPO receptors on cell surfaces

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If stem or progenitor cells are used for therapeutic treatments, then treatment effectiveness improves, but immune rejection increases

Engineering Contradiction:

Improvetreatment effectivenessVSAvoidimmune rejection

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies local quality by differentiating cell populations through controlled culture conditions to create specific cell subtypes with tailored properties that reduce immunogenicity while maintaining therapeutic function

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The patent uses preliminary action by pre-treating stem cells with vEPO protein variants and other modulators in the culture phase to prime cells for reduced immune recognition and enhanced survival after transplantation

Inventive Principle:

Principle #10Preliminary action

3Quantity of substance

If stem or progenitor cells are expanded in culture, then cell number increases, but endogenous factor production becomes uncontrolled

Engineering Contradiction:

Improvecell numberVSAvoidendogenous factor production

Core Design Contradiction:

Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent implements feedback control by monitoring and adjusting culture medium composition, including the addition of vEPO protein variants and growth factors, to maintain stable endogenous factor production while expanding cell numbers

Inventive Principle:

Principle #23Feedback

Solution Approach 2:

The patent changes chemical parameters of the culture environment by incorporating specific protein variants and growth factors that regulate endogenous factor production and prevent uncontrolled cellular behavior

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The vEPO protein variant improves the number, viability, and differentiation of stem and progenitor cells, facilitating their use in therapeutic applications by increasing their survival rate and enabling their effective transplantation for treating degenerative and inflammatory disorders.

Implementation Method 1

The vEPO protein variant is used in cell culture media to enhance the proliferation, survival, and differentiation of stem and progenitor cells by promoting a supportive environment for their expansion and function

Methodology Applied

Scientific EffectProtein-receptor binding:

Data Source

PatentUS8895303B2Method of cell culture and method of treatment comprising a vEPO protein variant

Publication Date: 2014.11.25 MEISEL ANDREAS

AI summary

In one aspect the present invention is concerned with a method of cell culture, comprising the steps of (i) obtaining a stem or progenitor cell sample, (ii) culturing the stem or progenitor cell sample in media and under closed conditions appropriate to cause proliferation or differentiation of the stem or progenitor cells, wherein the media comprises a vEPO protein variant, (iii) purifying the stem or progenitor cells ex vivo. The invention relates to a method of increasing the number and survival of stem and progenitor cells in vitro and in vivo using a vEPO protein variant. The invention also relates to improved differentiation of stem and progenitor cells in vitro and in vivo using a vEPO protein variant.