Vertically Oriented Matrix Holders for 3D Cell Culture Automation
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Solution Overview
Problem
Current three-dimensional cell culture systems are difficult to handle and automate, particularly those using scaffold-based matrices, which complicates the cultivation of animal and human cells in a physiologically relevant environment.
Innovation Solution
A cell culture device featuring vertically oriented matrix holders with central openings and a support system that allows for easy handling and automation, using hydrogels or porous support structures, enabling simultaneous cultivation of cells in different three-dimensional environments with automated medium changes and colonization processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If scaffold-based matrices are used for three-dimensional cell culture, then physiological relevance of cell cultivation is improved, but device complexity and ease of operation deteriorate
Solution Approach 1:
The device is divided into modular components: a support structure with multiple holders, each capable of independently accommodating different scaffold types (hydrogels, porous supports). This segmentation allows complex 3D culture functionality to be achieved through simple, interchangeable modules, reducing overall device complexity while maintaining physiological relevance.
Solution Approach 2:
The support structure is designed as a universal platform that can accommodate multiple types of scaffolds (hydrogels, porous support structures) and cell culture configurations simultaneously. Each holder is multi-functional, capable of supporting different matrix types and being used in various well strip configurations, thereby reducing the need for multiple specialized devices.
2Reliability
If scaffold-based matrices are used for three-dimensional cell culture, then physiological relevance of cell cultivation is improved, but ease of operation deteriorates
Solution Approach 1:
The holders are designed with reversible connection mechanisms to the support structure, allowing them to be easily attached and detached. This dynamic design enables operators to quickly change scaffold types and configurations without complex assembly procedures, significantly improving ease of operation while maintaining the ability to support physiologically relevant 3D structures.
3Productivity
If automated handling is implemented for cell culture devices, then productivity is improved, but device complexity increases
Solution Approach 1:
The modular holder design with standardized connection interfaces enables self-alignment and automatic recognition by robotic systems. The holders can be automatically transferred between positions without requiring complex positioning mechanisms or programming, allowing automated handling to improve productivity while adding minimal complexity to the device.
4Ease of operation
If vertically oriented matrix holders are used, then ease of operation and automation are improved, but manufacturing complexity increases
Solution Approach 1:
The vertically oriented holders are designed with uniform, simple geometries and homogeneous materials that are easy to manufacture using standard techniques. The consistent vertical orientation across all holders allows for standardized production processes, reducing manufacturing complexity despite the benefit of improved ease of operation and automation compatibility.
Data Source
AI summary
The invention relates to a cell culture device for a three-dimensional cell culture with one or more cell culture units, characterized in that a cell culture unit comprises:i. at least one matrix holder, with a central opening for uptake of a matrix;ii. a support, which is fixedly or reversibly connected to the at least one matrix holder; andiii. a well strip, consisting of a vessel with at least one cavity, which can comprise at least one matrix holder up to the upper end of the central opening or more,wherein the matrix holder is vertically oriented.