Biological Vesicle Detection via Enzyme-Substrate pH and Optical Changes
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Solution Overview
Problem
Current methods for liquid biopsy struggle to directly and effectively detect target marker molecules, such as proteins, on the surface of biological vesicles in body fluid-derived samples without pre-treatment, limiting their ability to quantify expression levels accurately.
Innovation Solution
A method and device that capture body fluid-derived samples on a support, using identification substances like antibodies to bind specifically to target marker molecules, and detect pH or optical changes generated by enzyme-substrate reactions, allowing for quantitative analysis of target marker molecules on the surface of biological vesicles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional detection methods (ELISA, FACS, NTA) are used to detect biological vesicles and target marker molecules, then detection capability is achieved, but preparation of measurement sample becomes complicated and miniaturization of measuring device becomes difficult
Solution Approach 1:
The invention extracts the detection function from complex conventional methods by using a simplified sensor system that directly detects pH changes or optical changes caused by enzyme-substrate reactions on captured biological vesicles, eliminating the need for complicated sample preparation and enabling device miniaturization
Solution Approach 2:
The invention replaces mechanical and complex optical detection systems with a simplified sensor-based system that detects biochemical reactions (pH changes or optical changes) directly, reducing device complexity while maintaining detection capability
2Measurement precision
If conventional detection methods are used to detect target marker molecules on biological vesicles, then detection is possible, but direct detection without pre-treatment cannot be achieved
Solution Approach 1:
The invention performs preliminary capture of biological vesicles on the support before detection, allowing direct detection of target marker molecules without subsequent pre-treatment steps, thereby simplifying operation while maintaining detection accuracy
Solution Approach 2:
The invention uses identification substances (antibodies) as intermediaries that specifically bind to target marker molecules on captured biological vesicles, enabling direct and specific detection without complex pre-treatment procedures
3Measurement precision
If multiple target marker molecules are detected on biological vesicles, then quantitative analysis capability is improved, but detection system complexity increases
Solution Approach 1:
The invention creates a universal detection platform where a single sensor system can detect multiple different target marker molecules by using different identification substances, enabling quantitative analysis of multiple markers without proportionally increasing system complexity
Solution Approach 2:
The invention segments the detection process into distinct functional modules (capture support, identification substance binding, signal detection), allowing independent optimization of each module and enabling multi-marker detection without overwhelming system complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables precise detection and measurement of target marker molecules, improving the quantitativity and efficiency of liquid biopsy by capturing multiple molecules on one support and counting biological vesicles with high expression levels with high precision.
Implementation Method 1
a step of detecting or measuring a pH change or an optical change generated by the identification substance bound to the target marker molecule
Implementation Method 2
a step of detecting or measuring a pH change or an optical change generated by the identification substance bound to the target marker molecule
Implementation Method 3
detect pH or optical changes generated by enzyme-substrate reactions
Implementation Method 4
a step of capturing a body fluid-derived sample containing a target marker molecule on a support
Data Source
AI summary
A method, a device, and a system for detecting or measuring a biological vesicle having a size of 20 microns or smaller, such as a cell, an exosome, or an extracellular vesicle among target substances detected by liquid biopsy, in a body fluid-derived sample and quantitatively analyzing the expression level of a target marker molecule, such as a protein, present on the surface thereof. A method for qualitatively or quantitatively analyzing the expression of a plurality of target marker molecules and a device and a system therefor could be provided by capturing a body fluid-derived sample containing multiple target marker molecules on a support and detecting or measuring the plurality of target marker molecules using an identification substance, such as an antibody.