Extracellular Vesicle Staining Compound for LC Size Measurement
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Solution Overview
Problem
Existing compounds used for staining extracellular vesicles are ineffective for size measurement by liquid chromatography, leading to inaccurate quantification.
Innovation Solution
A novel compound expressed by Formula (1) that conjugates with extracellular vesicles, enabling both staining and size measurement by liquid chromatography, characterized by specific alkyl, hydroxy, amino, or carboxy groups, and a linker formed of C, O, and N elements, allowing for covalent bonding with vesicles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Difficulty of detecting and measuring
If a compound is used for staining extracellular vesicles, then detection capability is improved, but size measurement capability deteriorates
Solution Approach 1:
The compound is divided into two distinct functional segments: a staining moiety (fluorescent group) for detection and a sizing moiety (size-standard-generating group) for size measurement. This segmentation allows each segment to independently perform its specific function without interfering with the other, resolving the contradiction between detection capability and size measurement capability
Solution Approach 2:
The compound is designed to perform multiple functions simultaneously: it acts as both a staining agent for fluorescent detection and a size standard generator for liquid chromatography measurement. The multi-functional design enables a single compound to satisfy both detection and size measurement requirements, eliminating the need for separate compounds for each purpose
2Difficulty of detecting and measuring
If existing staining compounds are used, then extracellular vesicle detection is enabled, but accurate quantification deteriorates
Solution Approach 1:
The compound pre-establishes a correlation between molecular weight and retention time through its sizing moiety before the actual measurement process. This preliminary encoding of size information into the compound's chromatographic behavior enables accurate quantification and size determination during subsequent liquid chromatography analysis, improving quantification accuracy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate size measurement and staining of extracellular vesicles, facilitating their detection and evaluation in various samples, including cosmetics, pharmaceutical products, foods, and biological tissues.
Implementation Method 1
a linker in which 0 to 12 chain molecules formed of elements selected from a group consisting of C, O, and N are bonded
Data Source
AI summary
An object is to provide a novel compound expressed by the following Formula (1) that conjugates with an extracellular vesicle.(in Formula (1), R1 represents H, a C1-C6 alkyl group, a hydroxy group, an amino group, or a carboxy group, R2 represents H, a C1-C18 alkyl group, a C1-C18 alkoxy group, NO2 or N(CH3)2, and R3 and R4 each independently represent H or CH3, where R3 and R4 may be bonded to each other to form a ring and, when R3 and R4 are bonded to each other to form a ring, R3 and R4 are CH2, “a” represents 0 or 1, and “b” represents 1, 2, or 3, and Z represents a linker in which 0 to 12 chain molecules formed of elements selected from a group consisting of C, O, and N are bonded, and the chain molecules may include a cyclic structure or a branched chain.)