Vibration Transmission Material for Cell Detachment
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Solution Overview
Problem
Existing cell culturing methods face challenges in maintaining a consistent temperature during cell detachment operations, which can lead to a decrease in cell survival rates due to temperature fluctuations.
Innovation Solution
A cell processing method and system that utilize a vibration unit to generate vibrations, where the vibration unit is configured to increase the temperature of a vibration transmission material by vibrating in a first mode, and then detach cells from a culture container by vibrating in a second mode, ensuring optimal temperature management.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If a culture container is taken out from an incubator for cell detachment operation, then the cell detachment operation can be executed, but the temperature of the cells becomes close to room temperature which lowers the survival rate of cells
Solution Approach 1:
The vibration transmission material is preheated to a temperature higher than the desired culture temperature before the culture container is placed on it. This preliminary heating action ensures that when the culture container is transferred for cell detachment, the heating material already maintains the container at the appropriate temperature, preventing temperature drop and maintaining high cell survival rate.
Solution Approach 2:
A vibration transmission material serves as an intermediary between the heating source and the culture container. This material not only transmits vibrations for cell detachment but also functions as a heating medium that can be preheated and then transfer thermal energy to the culture container, thereby maintaining temperature during the operation.
2Reliability
If a heater is arranged on a placement table to maintain culture medium temperature, then the temperature can be maintained, but the device complexity increases
Solution Approach 1:
The vibration transmission material serves multiple functions: it acts as a vibration transmission medium for cell detachment and simultaneously functions as a heating material that can be preheated and transfer thermal energy to the culture container. This multi-functionality eliminates the need for separate heating devices, thereby maintaining temperature reliability while avoiding increased device complexity.
Solution Approach 2:
The vibration transmission material is capable of self-heating through preheating and then automatically transfers this thermal energy to the culture container without requiring additional active control systems or complex heating apparatus. The material essentially serves itself as both the heating medium and the transmission medium.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for efficient temperature adjustment and cell detachment, maintaining high cell survival rates and proliferation by minimizing temperature fluctuations during the detachment process.
Implementation Method 1
making the vibration unit generate a vibration in a first mode in a state where the vibration unit makes contact with the vibration transmission material
Implementation Method 2
detaching the cells from the culture container by making the vibration unit generate a vibration in a second mode different from the first mode
Data Source
AI summary
A cell processing method for detaching cells adhering to a culture face of a culture container from the culture container by using a cell processing system including a vibration unit configured to generate vibrations includes heating a vibration transmission material to increase a temperature of the vibration transmission material by making the vibration unit generate a vibration in a first mode in a state where the vibration unit makes contact with the vibration transmission material, and detaching the cells from the culture container by making the vibration unit generate a vibration in a second mode different from the first mode after the heating is started, in a state where the vibration transmission material makes contact with the culture container.