Vibrational Energy for Enzyme Reaction and Protein Purification
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Solution Overview
Problem
Current methods for enhancing enzyme activity and protein purification are slow, cumbersome, and lack efficiency and cost-effectiveness, with a need for more efficient enzyme catalysis and protein purification techniques.
Innovation Solution
A method involving the combination of an enzyme and substrate to form an enzyme-substrate mixture, followed by mechanical mixing and the application of vibrational energy to accelerate enzymatic reactions, and a protein purification method using a protein binding film immobilized on a solid support for efficient separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional enzyme reaction methods are used, then the process is simple, but the reaction rate is slow and enzyme activity is low
Solution Approach 1:
The patent applies mechanical vibration to the enzyme-substrate mixture to accelerate enzymatic reactions. The vibration energy enhances the interaction between enzyme and substrate molecules, increasing reaction rates and enzyme activity without requiring complex chemical modifications or extreme conditions.
2Productivity
If traditional protein purification methods are used, then the process is conventional, but the method is slow and cumbersome
Solution Approach 1:
The patent employs mechanical vibration during protein purification processes to accelerate separation and binding kinetics. The vibration enhances the interaction between proteins and binding materials, reducing purification time while maintaining high purity levels, thereby resolving the contradiction between purification efficiency and time consumption.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Significantly enhances enzyme activity and reaction rates, reduces reaction times, and achieves high-purity protein purification with improved efficiency and cost-effectiveness.
Implementation Method 1
applying a vibrational energy to the enzyme-substrate mixture, thereby reacting the enzyme and the substrate
Implementation Method 2
contacting a protein mixture that includes the protein with a protein binding film, where the protein binding film is immobilized to a solid support, thereby forming a protein binding film complex
Data Source
Figure 1~2
Figure 3A~3B
Figure 3C~3D
AI summary
There are provided, inter alia, methods for reacting an enzyme and its substrate, methods for purifying a protein and an enzyme reactor and its use thereof.