Viral Protein Preparation Using Octylphenol-Free Detergents
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Solution Overview
Problem
The use of Triton X-100 in the manufacturing of viral vaccines is regulated due to its degradation product, octylphenol, which is an endocrine disruptor and harmful to aquatic wildlife, necessitating the need for sustainable alternatives.
Innovation Solution
The use of secondary alcohol ethoxylates, such as TERGITOL ™< 15-S-9 or DEVIRON ™< 13-S9, as detergents to disrupt virus particles and release viral proteins, replacing Triton X-100 in vaccine production processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If Triton X-100 is used to disrupt virus particles and release viral proteins, then the manufacturing efficiency and protein release are improved, but environmental harm and regulatory restrictions worsen due to octylphenol degradation products
Solution Approach 1:
The patent changes the chemical parameters of the detergent by replacing Triton X-100 with alternative detergents such as Tergitol 15-S-9, Devron 13-S9, and other non-ionic detergents that do not produce harmful degradation products. This substitution maintains the functional parameters (virus disruption and protein release efficiency) while changing the chemical composition to eliminate environmental harm from octylphenol.
2Ease of manufacture
If Triton X-100 is used as a detergent in vaccine manufacturing, then virus inactivation and protein solubilization are achieved, but the process becomes restricted by REACH regulations and requires exemptions
Solution Approach 1:
The patent changes the chemical identity parameter of the detergent from Triton X-100 to alternative detergents (Tergitol 15-S-9, Devron 13-S9, etc.) that are not restricted by REACH regulations. This maintains the functional parameters of virus inactivation and protein solubilization while improving regulatory compliance and eliminating the need for exemptions.
3Object-affected harmful factors
If alternative detergents are used to replace Triton X-100, then environmental sustainability is improved, but the effectiveness in disrupting virus particles and releasing proteins may worsen
Solution Approach 1:
The patent systematically evaluates and selects alternative detergents (Tergitol 15-S-9, Devron 13-S9, and other non-ionic detergents) that match the functional parameters of Triton X-100 in terms of virus disruption and protein release effectiveness, while improving the environmental sustainability parameter by eliminating octylphenol degradation products.
Solution Approach 2:
The patent identifies alternative detergents that copy the functional properties of Triton X-100 (non-ionic surfactants with similar HLB values and micelle formation characteristics) to ensure equivalent virus disruption and protein solubilization performance while avoiding the harmful degradation products.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach provides effective disruption and release of viral proteins while being environmentally friendly, maintaining vaccine efficacy and safety.
Implementation Method 1
subjecting a suspension of virions or a suspension of host cells to a detergent comprising a compound having a CAS registry number of CAS 68131-40-8 or CAS 84133-50-6, wherein each virion comprises a lipid membrane that comprises the at least one viral protein
Data Source
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AI summary
This application relates to methods for preparing viral proteins, such as influenza hemagglutinin proteins and/or influenza neuraminidase proteins, immunogenic compositions comprising the viral proteins thus obtained and vaccines comprising the same, as well as methods of using the same, in particular in the prevention and/or treatment of diseases or conditions caused by viruses, such as influenza viruses.