Viral 3′ UTR Regulatory Elements for ZCCHC2-Linked RNA Expression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The rapidly growing collection of viral sequences presents a significant challenge for functional annotation, as existing methods are inadequate for interpreting the vast number of viral sequences without clinical or industrial relevance, necessitating more effective strategies to identify regulatory elements for enhancing RNA stability or mRNA translation.
Innovation Solution
A method is developed to screen for regulatory elements using viral sequence data, discovering novel elements that interact with ZCCHC2, and utilizing these elements in constructs, vectors, or recombinant host cells to enhance RNA stability and mRNA translation, including a construct with a gene of a target protein in its 3′ UTR, and a composition involving ZCCHC2 interacting with these elements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If low-throughput analyses of pathogenic viruses are used, then functional annotation can be performed, but the vast number of viral sequences without clinical or industrial relevance cannot be effectively interpreted
Solution Approach 1:
The patent segments viral sequences into specific functional regions (such as 3' UTR regions) and focuses analysis on these segments rather than entire genomes. This allows high-throughput screening of regulatory elements while maintaining functional annotation accuracy for the most biologically relevant portions of viral sequences.
Solution Approach 2:
The patent applies local quality by focusing on specific functional regions of viral sequences (like 3' UTRs) that contain regulatory elements, rather than uniformly analyzing all sequence data. This prioritizes analysis of regions most likely to have biological activity, improving efficiency and relevance.
2Quantity of substance
If the entire virome is analyzed, then comprehensive viral sequence data is obtained, but functional annotation becomes intractable due to the vast number of sequences
Solution Approach 1:
The patent extracts and focuses on specific functional regions (3' UTRs and other regulatory elements) from the vast viral sequence data. By taking out only the biologically relevant portions, the patent reduces annotation complexity while maintaining comprehensive coverage of functional elements across the virome.
Solution Approach 2:
The patent identifies universal regulatory elements and motifs that can function across multiple viral families and species. This multi-functionality approach allows a single analytical framework to handle diverse viral sequences, reducing overall complexity while maintaining comprehensive functional annotation.
3Reliability
If novel regulatory elements are identified through viral sequence screening, then RNA stability and mRNA translation can be enhanced, but the complexity of analyzing and validating these elements increases
Solution Approach 1:
The patent performs preliminary computational screening and prediction of regulatory elements before experimental validation. By pre-identifying candidate elements through bioinformatic analysis of viral sequences, the patent reduces the complexity of subsequent experimental validation by focusing only on the most promising candidates.
Solution Approach 2:
The patent uses computational models and predictive algorithms as intermediaries between viral sequence data and functional validation. These intermediaries process and filter sequence data to identify likely regulatory elements, reducing the complexity of direct experimental analysis while maintaining reliability of the enhancement function.
Data Source
AI summary
The present disclosure relates to a novel regulatory element for enhancing RNA stability or mRNA translation; ZCCHC2 interacting with the regulatory element; and uses thereof. Being capable of increasing the expression of a target protein, the novel regulatory element for enhancing RNA stability or mRNA translation; and ZCCHC2 interacting with regulatory element according to the present disclosure are applicable in various fields, depending on the uses of the target protein.