Virtual Cellular Staining System for Crosstalk Reduction

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Solution Overview

Problem

Current imaging techniques involving multiplex staining face challenges in minimizing color crosstalk, requiring iterative trial and error processes that increase time and resource consumption.

Innovation Solution

A system and method for virtual cellular staining that allows users to simulate and visualize cell structures with different stain colors without actual reagents, using a processor, memory, and display to select and combine stain colors, reducing crosstalk and optimizing experiment setup.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional trial and error approach is used for multiplex staining, then crosstalk prevention can be achieved, but time consumption and resource usage significantly increase

Engineering Contradiction:
Improvecrosstalk preventionVSAvoidtime consumption
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by performing virtual staining simulations on digital cell images before conducting actual laboratory experiments. The system allows users to select different stain combinations and visualize their effects computationally, enabling optimization of staining protocols in advance. This preliminary computational step prevents crosstalk issues before they occur in real experiments, saving time and resources.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses copying by creating and manipulating digital copies of cell images through virtual staining. Instead of repeatedly performing physical staining experiments to test different combinations, the system creates virtual copies of cell images and applies digital stains to these copies. This allows multiple trial combinations to be tested computationally without consuming additional physical reagents or time.

Inventive Principle:
Principle #26Copying

2Reliability

If multiple reagents are purchased for multiplex staining optimization, then crosstalk can be minimized, but cost and resource consumption increase

Engineering Contradiction:
Improvecrosstalk minimizationVSAvoidreagent usage
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The system replaces physical reagents with digital stain representations. Virtual stains are applied computationally to digital cell image copies, eliminating the need to purchase and test multiple physical reagent combinations. This digital copying approach maintains the ability to optimize staining protocols while drastically reducing reagent consumption.

Inventive Principle:
Principle #26Copying

Solution Approach 2:

The system enables self-service by allowing users to independently optimize their staining protocols through virtual simulations. Users can select from available stain options, apply them virtually to their cell images, and evaluate the results without requiring physical reagents or laboratory equipment. This self-contained computational approach eliminates the need for iterative purchasing and testing of physical reagents.

Inventive Principle:
Principle #25Self-service

3Manufacturing precision

If iterative staining experiments are performed to optimize protocols, then optimal staining combinations can be found, but overall experiment time increases

Engineering Contradiction:
Improvestaining optimizationVSAvoidexperiment throughput
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The patent replaces the mechanical laboratory staining process with a computational system. Instead of physically applying stains to cells, performing washes, imaging, and analyzing results iteratively, the system uses digital image processing to simulate staining effects. This substitution of mechanical wet-lab procedures with computational operations maintains staining optimization capability while dramatically increasing experiment throughput.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The system performs preliminary computational optimization of staining protocols before actual experiments. By simulating different stain combinations on digital cell images in advance, users can identify optimal protocols without performing multiple iterative physical experiments. This preliminary computational action ensures staining optimization is achieved while maximizing subsequent laboratory productivity.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS9501844B2Virtual cellular staining
Publication Date: 2016.11.22 LIFE TECHNOLOGIES CORP
  • US9501844B2 patent drawing
  • US9501844B2 patent drawing
  • US9501844B2 patent drawing

AI summary

Systems and methods are used to display cell structures of a biological cell. A plurality of cell structures of a biological cell is stored and for each cell structure of the plurality of cell structures one or more stain colors are stored. A selected cell structure is received from an input device. One or more stain colors of the selected cell structure are retrieved. The one or more stain colors of the selected cell structure are displayed. A selected stain color is received from the input device. The selected cell structure is displayed in the selected stain color in an exemplary cell image. Further, a three-dimensional image of a biological cell is stored. The three-dimensional image is displayed on a display that includes a touch screen. A movement selection is received from the touch screen. The three-dimensional image is displayed on the display according to the movement selection.