Virtual Reference Value for Light Transmission Aggregometry
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Solution Overview
Problem
The collection of platelet-poor plasma (PPP) samples for light transmission aggregometry (LTA) measurements is stressful for patients, especially infants or those with limited blood volumes, and requires additional labor and consumables.
Innovation Solution
A method to create a database using light transmission measurements at multiple wavelengths on platelet-rich plasma (PRP) samples, establishing a correlation with PPP reference values, allowing the determination of a virtual reference value without needing an additional PPP sample.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If platelet-poor plasma (PPP) samples are collected for LTA measurements, then reliable reference values are obtained, but patient stress increases and additional labor and consumables are required
Solution Approach 1:
The patent creates a virtual PPP reference value by copying and processing data from PRP measurements taken at multiple wavelengths. Instead of physically collecting PPP samples, the system generates a virtual reference through mathematical processing of PRP spectral data, eliminating the need for additional blood collection while maintaining measurement reliability
Solution Approach 2:
The patent introduces a database and computational algorithm as an intermediary between PRP measurements and LTA reference values. This intermediary system processes multi-wavelength PRP data to generate virtual PPP references, replacing the direct physical measurement approach with an indirect computational method that avoids additional patient burden
2Measurement precision
If multiple light wavelengths are used for PRP measurements, then accurate virtual reference values are determined, but measurement complexity increases
Solution Approach 1:
The patent makes the light transmission measurement system multi-functional by using the same hardware setup for both PRP measurements and virtual PPP reference determination. The system performs multiple functions (spectral analysis, concentration determination, reference value generation) using a single integrated apparatus, reducing overall device complexity despite the sophisticated measurement protocol
Solution Approach 2:
The patent changes measurement parameters (light wavelengths) to resolve the contradiction. By measuring PRP at multiple specific wavelengths and using these parameter variations to calculate virtual PPP references, the system achieves high measurement precision while managing device complexity through standardized multi-parameter measurement protocols
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables reliable LTA measurements with reduced effort by using a virtual reference value derived from PRP measurements, eliminating the need for additional PPP samples.
Implementation Method 1
Performing a light transmission measurement with a first light wavelength and a second light wavelength different from the first on the PRP of the reference blood sample
Implementation Method 2
Due to their disc-shaped shape with a diameter of between 1.5 μm and 3 μm, light scattering occurs on the platelets, which makes the PRP appear as a cloudy liquid
Data Source
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AI summary
A method is disclosed for creating a database for determining a virtual reference value for a light transmission aggregometry measurement, comprising the following steps: a. providing a platelet-rich plasma "PRP" (17) of a reference blood sample; b. carrying out a light transmission measurement on the PRP (17) of the reference blood sample at a first light wavelength and a second light wavelength (14), which differs from the first; c. providing a platelet-poor plasma "PPP" of the reference blood sample; d. carrying out a light transmission measurement on the PPP to determine a PPP reference value; e. assigning the measurement result of step d to the measurement results of step b in a database; f. repeating steps a to e for a plurality of reference blood samples. The database obtained by this method allows the determination of a virtual reference value which represents an excellent estimator for the PPP reference value. After the database has been created, it is possible to determine virtual reference values of blood samples to be examined, and LTA measurements can be carried out using the virtual reference values without needing to obtain PPP of the blood samples to be examined.