Multidimensional Chromatography for Rapid Vitamin D Detection
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Solution Overview
Problem
Conventional methods for extracting highly lipophilic components, such as vitamin D, from oil or fat and biological samples are cumbersome, require large amounts of solvent, and face challenges in switching between chromatography analyses, leading to inefficient and inconvenient processes.
Innovation Solution
A multidimensional chromatography system combining supercritical chromatography and reverse phase liquid chromatography, with a multidimensional chromatography system that includes a supercritical chromatography part and a reverse phase liquid chromatography part, allowing for direct switching between columns and reducing the need for solvent-based pretreatment, using a modifier like methanol to facilitate the separation and detection of vitamin D.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional solvent extraction methods are used to separate vitamin D from oil or fat, then vitamin D can be extracted, but the process requires large amounts of solvent and is time-consuming
Solution Approach 1:
The patent replaces conventional mechanical solvent extraction with supercritical fluid chromatography, where supercritical carbon dioxide serves as the mobile phase to extract and separate vitamin D from lipid samples, eliminating the need for large amounts of organic solvent while maintaining extraction efficiency
Solution Approach 2:
The patent changes the physical state and properties of the mobile phase by using supercritical fluid (carbon dioxide at elevated temperature and pressure) instead of conventional liquid solvents, allowing for efficient extraction and separation with reduced solvent consumption and faster analysis
2Measurement precision
If normal phase liquid chromatography is used to wash extracted vitamin D, then vitamin D can be separated, but impurities accumulate on the chromatography column reducing its lifetime
Solution Approach 1:
The patent changes the chromatography mode from normal phase to reverse phase liquid chromatography for the washing step, where the reversed polarity of the stationary phase prevents impurity accumulation while maintaining effective separation of vitamin D from lipid components
Solution Approach 2:
The patent introduces a reverse phase chromatography column as an intermediary step between the supercritical fluid extraction and the final analysis, serving as a protective barrier that prevents impurities from damaging the main chromatography column while still enabling effective vitamin D separation
3Extent of automation
If two-dimensional column switching system with reverse phase liquid chromatography is used, then vitamin D can be analyzed at online, but the system complexity increases
Solution Approach 1:
The patent merges supercritical fluid chromatography and reverse phase liquid chromatography into a unified multidimensional chromatography system with automated column switching, enabling online analysis while managing system complexity through integrated control and coordination of the two chromatography modules
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables rapid, accurate, and automated analysis of highly lipophilic components like vitamin D, improving analysis efficiency, reducing costs, and providing high reproducibility and recovery rates.
Implementation Method 1
a supercritical chromatography column for removing nonpolar substances from the sample
Implementation Method 2
a reverse phase liquid chromatography column for separating weakly polar substances
Data Source
AI summary
The present invention relates to an analysis system that is applied to rapid automatic analysis and detection of highly lipophilic components and a method for rapidly detecting vitamin D in an oil or fat, or a biological sample. In the present invention, online pretreatment and separation of a sample containing highly lipophilic components in a sample to be analyzed are performed by a multidimensional chromatography system. The multidimensional chromatography system includes a supercritical chromatography part and a reverse phase liquid chromatography part sequentially connected, the reverse phase liquid chromatography part including one or more reverse phase liquid chromatography columns, and the supercritical chromatography part including a supercritical mobile phase, a modifier, and a supercritical packed column.