Vitamin D Sensor Chip Using Oxidoreductase Enzyme
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Solution Overview
Problem
Current methods for measuring 25-hydroxyvitamin D concentrations are time-consuming, costly, and prone to measurement errors, making them suboptimal for clinical examinations.
Innovation Solution
A novel quantification method involving the use of an oxidoreductase, such as cholesterol oxidase or glucose-methanol-choline family oxidoreductase, which reacts with vitamin D derivatives to determine their concentration through enzymatic reactions, utilizing a sensor chip and electrode for accurate and efficient measurement.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If immunoassay methods (ECLIA, CLEIA, CLIA) are used to measure 25-hydroxyvitamin D, then measurement sensitivity is improved, but measurement time and cost increase
Solution Approach 1:
The patent replaces the mechanical/chemical immunoassay system with an electrochemical sensor system. The sensor uses an electrode with a membrane containing 25-hydroxyvitamin D-binding protein to directly detect vitamin D levels through electrical signals, eliminating the need for complex immunoassay procedures while maintaining measurement capability.
Solution Approach 2:
The patent introduces 25-hydroxyvitamin D-binding protein as an intermediary substance in the membrane layer. This binding protein specifically binds to 25-hydroxyvitamin D in the sample, enabling selective detection and providing the necessary sensitivity without requiring the complex antibody-based immunoassay systems.
2Measurement precision
If immunoassay methods are used to measure 25-hydroxyvitamin D, then measurement sensitivity is improved, but measurement cost increases
Solution Approach 1:
The patent employs a disposable sensor chip design where the electrode with the protein-containing membrane is used once and then discarded. This eliminates the need for expensive, reusable immunoassay equipment and reagents, significantly reducing per-test costs while maintaining detection sensitivity through the specialized membrane design.
Solution Approach 2:
The use of 25-hydroxyvitamin D-binding protein as a mediator in the membrane provides specific binding capability at low cost, replacing the expensive monoclonal antibodies used in immunoassays. This intermediary enables selective detection without the high reagent costs associated with traditional immunoassay methods.
3Productivity
If conventional measurement methods are used, then comprehensive analysis is provided, but measurement accuracy decreases due to errors
Solution Approach 1:
The patent replaces conventional multi-step measurement methods with a direct electrochemical detection system. The sensor provides comprehensive vitamin D analysis through a single measurement process, eliminating multiple handling steps that introduce errors in traditional methods.
Solution Approach 2:
The sensor system performs self-calibration and automatic measurement without requiring complex manual procedures. The binding protein in the membrane automatically binds to vitamin D in the sample, and the electrochemical system directly reads the concentration, minimizing human intervention and associated measurement errors.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides a faster, more cost-effective, and accurate means of quantifying 25-hydroxyvitamin D levels, reducing labor and error, and is suitable for clinical use.
Implementation Method 1
adding an oxidoreductase to a sample; the oxidoreductase may be an oxidase, and hydrogen peroxide produced or oxygen consumed by adding the oxidase may be quantified
Data Source
AI summary
A quantitation method of vitamin D derivative is provided including adding an oxidoreductase to a sample. The quantitation method of vitamin D derivative may further include reducing a mediator by adding the oxidoreductase, and reacting the reduced mediator with a reagent to determine a concentration of the vitamin D derivative.