Vitrification Cryopreservation Device with Absorber
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Solution Overview
Problem
Current methods for vitrification cryopreservation face challenges such as long freezing times, limited capacity for large cell or tissue samples, complexity in removing excess vitrification solution, and poor handleability, especially when dealing with sheet-like tissues or cell sheets.
Innovation Solution
A device for vitrification cryopreservation featuring a support with a vitrification solution absorber that includes an adhesive layer and a vitrification solution absorbing layer, specifically designed to absorb excess vitrification solution and provide excellent visibility, using materials like paper, nonwoven fabric, porous resin sheets, or porous metal oxide sheets to facilitate efficient cryopreservation and thawing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If slow freezing method is used, then cells or tissues can be preserved, but the procedure takes a long time and requires temperature-controlling devices
Solution Approach 1:
The patent changes the cooling rate parameter from slow (0.3-0.5°C/min) to rapid (direct contact with liquid nitrogen at -196°C), and modifies the solution composition by using vitrification solution with high concentration cryoprotectant instead of standard preservation solution, enabling quick freezing without temperature-controlling devices
2Reliability
If slow freezing method is used, then cells or tissues can be preserved, but ice crystals form outside the cells causing physical damage
Solution Approach 1:
The patent changes the solution composition parameter by using vitrification solution with high concentration cryoprotectant (20-80% v/v), which depresses the freezing point and prevents ice crystal formation during rapid cooling, eliminating the harmful effect of ice crystals on cells and tissues
3Object-affected harmful factors
If vitrification solution with large amount of cryoprotectant is used, then ice crystals are prevented, but the solution is chemically toxic and requires minimal volume and short exposure time
Solution Approach 1:
The patent applies partial action by using a paper substrate that absorbs excess vitrification solution, leaving only the necessary amount in contact with the cells or tissues. This reduces the volume of toxic solution and exposure time while maintaining the ice crystal prevention effect
4Area of stationary object
If excess vitrification solution surrounds the cells or tissues, then complete coverage is achieved, but the solution needs to be removed which complicates the procedure
Solution Approach 1:
The patent employs self-service by using a paper substrate that automatically absorbs and removes excess vitrification solution through capillary action, eliminating the need for additional removal steps such as suction or wiping, and simplifying the overall procedure
5Area of stationary object
If sheet-like tissues or cell sheets are cryopreserved, then large samples can be preserved, but handleability and removal of excess solution become difficult
Solution Approach 1:
The patent employs self-service by using a paper substrate that automatically absorbs and removes excess vitrification solution through capillary action, eliminating the need for additional removal steps such as suction or wiping, and simplifying the overall procedure
Solution Approach 2:
The patent uses a flexible paper substrate that can conform to and support sheet-like tissues and cell sheets, providing mechanical support and ease of handling while maintaining the self-absorbing function for excess solution removal
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables easy and reliable cryopreservation of cells or tissues with improved visibility, allowing for quick freezing and thawing without the need for additional procedures, and is suitable for both small and large samples, including sheet-like tissues.
Implementation Method 1
a vitrification solution absorbing layer (3) made of at least one selected from paper, nonwoven fabric, porous resin sheets, and porous metal oxide sheets
Implementation Method 2
a vitrification solution absorber including at least an adhesive layer (5) and a vitrification solution absorbing layer (3) in the given order on the support (4)
Implementation Method 3
the device including a portion with no adhesive layer disposed between the support and a portion of the vitrification solution absorbing layer configured to hold a cell or tissue
Data Source
AI summary
The present invention aims to provide a device for vitrification cryopreservation enabling easy and reliable vitrification cryopreservation of a cell or tissue. The device for vitrification cryopreservation of a cell or tissue of the present invention includes a support and a vitrification solution absorber including at least an adhesive layer and a vitrification solution absorbing layer in the given order on the support. The device includes a portion with no adhesive layer disposed between the support and a portion of the vitrification solution absorbing layer configured to hold a cell or tissue.