Volumetric Imaging with Continuous Axial Scanning
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Solution Overview
Problem
Existing methods for three-dimensional imaging of biological samples with sub-micrometer resolution are slow and labor-intensive due to discrete imaging operations and system settling times.
Innovation Solution
Continuous movement of the sample along the optical axis while imaging, combined with simultaneous image acquisition using an imaging module, enhances throughput by reducing waiting times and optimizing sensor readout.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If discrete imaging operations are used to maintain high resolution, then measurement precision is improved, but productivity deteriorates due to slow acquisition speed
Solution Approach 1:
The patent implements continuous motion of the objective lens or sample stage during image acquisition, eliminating discrete stop-and-go imaging operations. The sensor continuously integrates photons while the system moves, allowing volumetric imaging to proceed without interruption. This continuous action maintains sub-micrometer resolution through controlled motion while dramatically increasing throughput by removing settling times and discrete operation overhead.
Solution Approach 2:
The system transitions from static, discrete imaging to dynamic continuous imaging. The objective lens or sample stage moves continuously during acquisition, and the sensor readout operates continuously rather than in discrete frames. This dynamic approach allows the system to capture volumetric data at high speed while maintaining resolution through synchronized motion control and continuous sensor integration.
2Reliability
If the system stops to settle between imaging operations to maintain stability, then reliability is improved, but loss of time increases due to waiting periods
Solution Approach 1:
The system eliminates settling periods by implementing continuous motion and continuous sensor integration. The objective lens or sample stage moves without stopping, and the sensor continuously accumulates photons throughout the volumetric imaging process. This removes all waiting and settling time, achieving rapid volumetric acquisition while maintaining image quality through controlled continuous motion rather than discrete stabilized positions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases imaging throughput while maintaining high resolution, allowing for rapid acquisition of volumetric images with improved efficiency.
Implementation Method 1
an objective lens configured to transmit photons from one or more object planes within the sample to one or more sensors
Data Source
AI summary
The present disclosure provides a methods and systems for analyzing one or more samples. The method may comprise using a plurality of sensors to substantially continuously integrate through a plurality of object planes of the sample. The integration can then generate a volumetric measurement of the sample.