Volumetric Imaging With Continuous Objective Motion
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Solution Overview
Problem
Existing methods for three-dimensional imaging of biological samples with sub-micrometer resolution are slow and labor-intensive due to the need for discrete imaging operations and system settling times, which significantly reduce overall image acquisition throughput.
Innovation Solution
A method and system for volumetric imaging that involves continuously moving the objective lens relative to the sample while simultaneously acquiring a series of images, allowing for high-resolution imaging with increased throughput by reducing waiting times and optimizing sensor readout.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If discrete imaging operations are performed with system settling time between operations, then high resolution imaging is achieved, but image acquisition throughput is reduced by a factor of two or more
Solution Approach 1:
The patent implements continuous motion of the objective lens or sample stage without discrete stopping and settling operations. The imaging system continuously acquires images while moving, eliminating idle settling time between imaging operations. This continuous operation mode maintains sub-micrometer resolution while doubling the image acquisition throughput by keeping the system in constant productive operation without interruption.
2Measurement precision
If the sensor waits for full readout before acquiring the next image, then image quality is maintained, but system throughput is significantly reduced
Solution Approach 1:
The patent initiates sensor readout operations in advance before the actual imaging exposure is complete. By overlapping the readout process with the ongoing imaging acquisition, the system prepares the next image data structure and begins transferring data while previous data is still being captured. This preliminary action eliminates idle wait time between images while preserving image quality through proper data sampling and processing timing.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances imaging throughput by up to a factor of two while maintaining high resolution, enabling rapid acquisition of detailed biological sample information, including the location and presence of analytes within the sample.
Implementation Method 1
an objective lens configured to transmit photons from one or more object planes within the sample to one or more sensors
Data Source
AI summary
The present disclosure provides a methods and systems for analyzing one or more samples. The method may comprise using a plurality of sensors to substantially continuously integrate through a plurality of object planes of the sample. The integration can then generate a volumetric measurement of the sample.