Volumetric Imaging With Continuous Objective Scanning
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Solution Overview
Problem
Existing methods for three-dimensional imaging of biological samples with sub-micrometer resolution are slow and labor-intensive due to the need for discrete imaging operations and waiting for the system to settle and move, significantly reducing overall image acquisition throughput.
Innovation Solution
A method and system for volumetric imaging that involves continuously moving the objective lens relative to the sample while simultaneously acquiring a series of images, allowing for high-resolution imaging with increased throughput by reducing the time spent waiting for sensor readout.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If discrete imaging operations are performed with waiting for system settlement and movement, then measurement precision is maintained, but productivity deteriorates
Solution Approach 1:
The patent implements continuous scanning of the objective lens through the sample volume while continuously acquiring images, eliminating the discrete stop-and-wait operations. The lens moves continuously along the optical axis and the sensor continuously reads out image data, maintaining useful action throughout the imaging process rather than pausing for settlement between discrete imaging operations.
Solution Approach 2:
The system transitions from static discrete imaging to dynamic continuous scanning. The objective lens is moved dynamically through the sample volume during image acquisition, and the sensor operates in a dynamic continuous readout mode rather than static frame-by-frame capture, enabling simultaneous movement and imaging.
2Volume of moving object
If the objective lens is moved between imaging operations, then volumetric coverage is improved, but loss of time increases due to settling and movement waiting
Solution Approach 1:
The patent eliminates idle time by making the lens movement and image acquisition simultaneous continuous operations. The objective lens moves continuously through the sample volume while the sensor continuously captures images, so time is not lost waiting for movement or settlement between imaging operations.
Solution Approach 2:
The system prepares for continuous imaging by coordinating the lens movement speed with the sensor readout rate in advance, ensuring that images are acquired at the optimal moments during continuous scanning without requiring intermediate settlement periods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances imaging throughput by maintaining high resolution and reducing the time required for image acquisition, enabling rapid and efficient volumetric imaging of biological samples.
Implementation Method 1
an objective lens configured to transmit photons from one or more object planes within the sample to one or more sensors
Data Source
AI summary
The present disclosure provides a methods and systems for analyzing one or more samples. The method may comprise using a plurality of sensors to substantially continuously integrate through a plurality of object planes of the sample. The integration can then generate a volumetric measurement of the sample.