vWF-cleaving protease detection via ELISA antibody binding
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Solution Overview
Problem
Current methods for detecting thrombosis and thrombophilia related to platelet aggregation are complex, time-consuming, and not commonly used in clinical settings, lacking a convenient and accurate approach for diagnosis and treatment.
Innovation Solution
A method and kit using monoclonal or polyclonal antibodies to measure the concentration of von Willebrand factor-cleaving protease (vWF-cleaving protease) in plasma, allowing for rapid and specific detection of thrombosis or thrombophilia through enzyme-linked immunosorbent assay (ELISA) or other immunological methods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods (SDS-agarose electrophoresis and autoradiography or Western blotting) are used to measure vWF-cleaving protease activity, then measurement can be performed, but the procedure is complex, time-consuming (3 days), and not commonly used in clinical settings
Solution Approach 1:
The patent extracts the essential measurement function from complex electrophoresis and blotting procedures by using an ELISA-based method that directly measures vWF-cleaving protease activity in plasma samples without requiring protein separation or transfer steps, reducing measurement time from 3 days to a single day while maintaining measurement capability
Solution Approach 2:
The patent replaces the mechanical electrophoresis separation system with an immunological detection system (ELISA) that uses antibody-antigen binding to detect and quantify vWF-cleaving protease activity, eliminating the need for complex electrophoresis apparatus and reducing procedural complexity
2Measurement precision
If conventional methods (SDS-agarose electrophoresis and autoradiography or Western blotting) are used to measure vWF-cleving protease, then measurement can be performed, but the procedure contains complicated steps and measured values often vary in accordance with laboratories
Solution Approach 1:
The patent creates a universal measurement method using ELISA that can be performed in any laboratory with standard ELISA equipment and reagents, eliminating the need for specialized electrophoresis and blotting facilities, thereby reducing procedural complexity and improving inter-laboratory consistency while maintaining measurement precision
Solution Approach 2:
The patent changes the measurement parameter from detecting large vWF multimers (which requires electrophoresis separation) to directly measuring vWF-cleving protease activity through antibody-antigen binding in ELISA, simplifying the measurement procedure and reducing variability between laboratories
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables convenient, rapid, and reproducible detection of thrombosis or thrombophilia, reducing measurement time from days to hours and improving diagnostic accuracy compared to conventional methods.
Implementation Method 1
measuring a von Willebrand factor-cleaving protease... using an immunological method with a monoclonal antibody and/or a polyclonal antibody against the von Willebrand factor cleaving protease
Data Source
AI summary
A method of detecting thrombosis or the degree of thrombophilia by measuring a von Willebrand factor cleaving protease, and a kit for detecting thrombosis or the degree of thrombophilia, comprising an antibody or a fragment thereof specifically binding to a von Willebrand factor-cleaving protease, are disclosed. The detection method and the detection kit have an excellent convenience, rapidity, and specificity.