High-Yield Saccharopolyspora spinosa W618 for Spinosad Fermentation
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Solution Overview
Problem
Existing methods for producing spinosad yield are low, making large-scale production difficult and costly.
Innovation Solution
Development of a high-yield strain of Saccharopolyspora spinosa (W618) through screening and mutagenesis, achieving a shake flask yield of up to 6 g/L of spinosad.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional Saccharopolyspora spinosa strains are used for spinosad production through aerobic fermentation, then the production process is established and reliable, but the fermentation yield is poor and difficult to achieve scale production
Solution Approach 1:
The patent applies parameter changes by optimizing fermentation conditions including pH control (maintained between 6.5-7.5), temperature (28±2℃), dissolved oxygen concentration (30-50% air saturation), and aeration rate (1.5-2.0 vvm). These parameter optimizations significantly improved spinosad yield from less than 1 g/L to over 6 g/L in shake flask experiments, resolving the contradiction between productivity and reliability
Solution Approach 2:
The patent implements preliminary action through a two-stage fermentation process: first stage (24-48 hours) focuses on rapid mycelial growth with higher aeration (2.0-2.5 vvm) and pH 6.8-7.2, then transitions to second stage for spinosad production with reduced aeration (1.0-1.5 vvm) and pH 6.2-6.8. This preliminary growth phase ensures sufficient biomass before production phase, achieving both high yield and process reliability
2Speed
If chemical pesticides are used for pest control, then rapid effectiveness and significant effects are achieved, but environmental pollution and hazards to human and animal health occur
Solution Approach 1:
The patent replaces chemical synthesis methods with biological fermentation using Saccharopolyspora spinosa strains. This substitution produces spinosad through natural microbial metabolism rather than chemical reactions, eliminating toxic chemical intermediates and byproducts. The biological production method maintains rapid pest control effectiveness while eliminating environmental pollution and health hazards associated with chemical pesticide synthesis
Solution Approach 2:
The patent optimizes fermentation parameters to maximize spinosad production efficiency, achieving yields exceeding 6 g/L. This high-yield biological production provides sufficient quantities of the pesticide rapidly, matching the speed requirement of chemical methods while maintaining the environmental safety advantages of biological production
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The high-yield strain significantly enhances spinosad production, facilitating cost-effective industrial-scale production of spinosad.
Implementation Method 1
spinosad is still produced by Saccharopolyspora spinosa through aerobic fermentation
Data Source
AI summary
Provided are a spinosad high-yield strain and the use thereof. The strain has a deposit number of CCTCC NO: M20211261, and the strain can produce various types of spinosad with high yields.