Washed Platelet Extract for Reduced Immune Reaction Risk
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Solution Overview
Problem
Current platelet-rich plasma (PRP) and platelet lysate (PL) formulations have limitations, including high donor serum requirements, low growth factor and cytokine concentrations, and risks of serum sickness and allergic reactions due to immunogenic plasma proteins, which restrict their clinical effectiveness in human and veterinary medicine.
Innovation Solution
A method for producing a washed platelet extract by separating plasma from whole blood, washing platelets with buffered saline, lysing them with a detergent solution to form a platelet lysate, and removing insoluble components, resulting in a composition free of plasma serum proteins and enriched with growth factors, which can be lyophilized for storage and use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If platelet-rich plasma (PRP) or platelet lysate (PL) is used for regenerative medicine, then growth factors and cytokines are delivered to promote tissue regeneration, but plasma proteins cause immune reactions and serum sickness
Solution Approach 1:
The patent extracts and removes plasma proteins from the platelet lysate through a washing step using buffered saline. This separation isolates the beneficial growth factors and cytokines while eliminating the harmful immunogenic plasma proteins, directly resolving the contradiction between delivering regenerative factors and avoiding immune reactions
Solution Approach 2:
The patent changes the composition parameters of the platelet lysate by adjusting protein concentration through washing. By reducing plasma protein content while maintaining growth factor concentrations, the formulation achieves both safety from immune reactions and effectiveness for tissue regeneration
2Quantity of substance
If standard platelet lysate preparation is used, then platelets are disrupted to release growth factors, but the concentration of growth factors and cytokines remains low
Solution Approach 1:
The washing step extracts and removes plasma proteins while concentrating the platelet-derived growth factors and cytokines in the remaining supernatant. This extraction process increases the relative concentration of beneficial factors, directly addressing the low concentration problem
Solution Approach 2:
The patent optimizes the concentration parameters by controlling the washing volume and centrifugation conditions. This achieves higher growth factor concentrations in the final formulation, improving both quantity of active substances and clinical productivity
3Quantity of substance
If large volumes of donor serum are used in PRP formulations, then sufficient growth factors are available, but the volume is too large for injection into anatomical regions
Solution Approach 1:
The washing step extracts plasma proteins and reduces the serum volume while retaining growth factors. This concentration process allows delivering sufficient growth factors in a smaller, clinically acceptable injection volume
Solution Approach 2:
The patent changes the volume parameter by reducing serum content through washing while maintaining or increasing growth factor concentration. This achieves the dual goal of sufficient growth factor delivery and reduced injection volume for anatomical feasibility
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The washed platelet extract achieves a higher concentration of growth factors and cytokines while minimizing the risk of immune reactions, allowing for effective treatment of injuries and conditions such as lameness and tendon injuries with reduced risk of serum sickness.
Implementation Method 1
adding a detergent solution comprising between 0.1% and 2.5% detergent to the washed platelets and incubating the washed platelets with the detergent solution for a time sufficient to lyse at least 50% of the platelets
Implementation Method 2
separating plasma from a whole blood sample by spinning the whole blood sample at a speed between 600×g and 1000×g for about 5 minutes
Implementation Method 3
removing residual detergent from the washed platelet extract by spinning or using hydrophobic interaction chromatography
Implementation Method 4
lyophilizing the washed platelet extract to form a lyophilized washed platelet extract
Data Source
AI summary
A washed platelet extract with increased platelet growth factor concentration and decreased total protein relative to platelet rich plasma, and methods of making and using such an extract for the treatment of disease and injuries recited herein, are provided.