Water Toxicity Analysis Device with Parallel Measurement Chambers

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Solution Overview

Problem

Existing water toxicity monitoring systems are limited by their inability to analyze multiple samples simultaneously with high precision and accuracy, and they require significant time for analysis, making them unsuitable for rapid intervention in unexpected water contamination events. Additionally, these systems are prone to cross-contamination and fouling due to hydraulic handling of microorganisms.

Innovation Solution

A device based on random access technology that allows for continuous, autonomous monitoring and analysis of multiple samples simultaneously, using a reaction tray with luminometric and fluorimetric modules for rapid measurement and a mechanical arm for precise handling and washing, reducing the risk of contamination and fouling.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Extent of automation

If flow technology analyzers are used for automated toxicity monitoring, then automation is achieved, but multiple samples cannot be analyzed simultaneously and analysis time is extended

Engineering Contradiction:
Improveautomated toxicity monitoringVSAvoidsamples analyzed per unit time
Core Design Contradiction:
Extent of automationVSProductivity

Solution Approach 1:

The device divides the analysis system into multiple independent measurement chambers (at least two chambers) that can simultaneously analyze different samples. Each chamber operates independently with its own flow path, allowing parallel processing of multiple samples without cross-contamination, thus increasing productivity while maintaining automation.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention transitions from sequential single-sample analysis to parallel multi-sample analysis by adding spatial dimensionality through multiple measurement chambers. This dimensional expansion allows simultaneous analysis of multiple samples along the time axis, resolving the contradiction between automation and productivity.

Inventive Principle:
Principle #17Another dimension (Dimensionality change)

2Extent of automation

If hydraulic handling of microorganisms is used in flow analyzers, then automated analysis is achieved, but cross-contamination and fouling occur

Engineering Contradiction:
Improveautomated sample handlingVSAvoidcross-contamination resistance
Core Design Contradiction:
Extent of automationVSReliability

Solution Approach 1:

The system segments the hydraulic flow paths into separate, isolated channels for each measurement chamber. Each chamber has its own dedicated inlet and outlet ports, preventing cross-contamination between samples while maintaining automated hydraulic handling. The physical separation ensures that microorganisms in different chambers cannot mix.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention introduces separate hydraulic intermediaries (individual pumps and flow control mechanisms) for each measurement chamber. These intermediaries manage the hydraulic handling of microorganisms independently, ensuring automated operation while preventing fouling and cross-contamination through isolated flow paths.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Measurement precision

If manual analysis methods are used with luminometers, then measurement precision is maintained, but analysis time is extended and operator involvement is required

Engineering Contradiction:
Improvetoxicity measurement accuracyVSAvoidanalysis time per sample
Core Design Contradiction:
Measurement precisionVSLoss of time

Solution Approach 1:

The invention merges multiple measurement chambers into a single integrated device with unified control systems. This consolidation allows simultaneous analysis of multiple samples while maintaining the precision of individual luminometric measurements. The combined system eliminates the need for sequential manual analysis, reducing total analysis time while preserving measurement accuracy through standardized measurement protocols.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The device enables continuous automated analysis by maintaining constant flow of microorganisms through multiple measurement chambers. The hydraulic system ensures uninterrupted measurement sequences, eliminating idle time between samples while preserving measurement precision through consistent flow conditions and standardized measurement protocols.

Inventive Principle:
Principle #20Continuity of useful action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables rapid analysis of tens of samples with high precision and accuracy, providing real-time contamination alerts and extended usability of microbic substrates, while minimizing cross-contamination and fouling risks, and allowing for simultaneous evaluation of toxicity effects on both bacteria and algae.

Implementation Method 1

measuring bioluminescence variations of bacteria rehydrated from lyophilized lots before the analytical tests

Methodology Applied
Scientific EffectBioluminescence: Bioluminescence

Implementation Method 2

The monitored phenomenon is the algal delay in the light emission, better known as delayed fluorescence, caused by phytotoxic interfering substances

Methodology Applied
Scientific EffectDelayed fluorescence: Fluorescence

Data Source

PatentUS9927418B2Device and method for the determination and monitoring of water toxicity
Publication Date: 2018.03.27 MOSCETTA POMPEO
  • US9927418B2 patent drawing
  • US9927418B2 patent drawing
  • US9927418B2 patent drawing

AI summary

A device for analysis and monitoring of toxicity in waters is described. The device is specifically devised for determining toxicity in waters in several samples at the same time and in quick time with a high degree of accuracy and precision. The device has application in the field of controlling and monitoring the water resources and in the field of the ecotoxicological analyses.