Wavelength Multiplexed Fourier Ptychographic Microscopy
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Solution Overview
Problem
The existing Fourier ptychographic microscopy (FPM) method is limited by a long data collection time, which prevents the observation of dynamic sample activities, thereby restricting its application in biological imaging due to the trade-off between high spatial resolution and temporal resolution.
Innovation Solution
A wavelength multiplexed Fourier ptychographic microscopy system utilizing a three-color LED array and an RGB camera, synchronized by a controller, to rapidly collect and process multiple low-resolution images across different wavelengths, enabling the restoration of high-resolution intensity and phase images with reduced collection time.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If sequential illumination strategy is used to collect multiple low-resolution images, then high spatial resolution can be achieved through Fourier ptychography, but data collection time becomes excessively long (several minutes for 200+ images)
Solution Approach 1:
The patent merges multiple illumination wavelengths (red, green, blue LEDs) into a single simultaneous illumination event, allowing the collection of multiple low-resolution images at different wavelengths in parallel rather than sequentially. This reduces the data collection time from several minutes to a fraction of a second while maintaining the ability to reconstruct high-resolution images through Fourier ptychographic algorithms.
Solution Approach 2:
The patent introduces wavelength as an additional dimension to the illumination strategy. Instead of varying illumination angle sequentially in time, the system simultaneously varies illumination wavelength across three color channels, effectively adding a spectral dimension that enables parallel data collection while maintaining spatial frequency information for high-resolution reconstruction.
2Measurement precision
If sequential illumination of multiple LED sources is used, then high spatial resolution images can be reconstructed, but temporal resolution is sacrificed making dynamic sample activity unobservable
Solution Approach 1:
The patent combines multiple wavelength illuminations into a single simultaneous illumination event, enabling the capture of dynamic sample activity across different wavelengths at the same time point. This maintains temporal resolution while preserving the ability to reconstruct high-resolution images through computational methods.
Solution Approach 2:
The patent implements continuous simultaneous illumination across multiple wavelength channels, eliminating the interruptions and sequential steps required in traditional FPM. This continuous illumination enables observation of dynamic biological processes in real-time while maintaining high spatial resolution through the reconstructed image data.
3Device complexity
If a single LED array is used for illumination, then the system structure remains simple, but the ability to simultaneously capture multi-wavelength information is limited
Solution Approach 1:
The patent segments the illumination system into three distinct LED arrays (red, green, blue) that can be independently controlled and activated simultaneously. This segmentation allows each LED to illuminate the sample at different wavelengths at the same time, enabling parallel multi-wavelength image collection while keeping each individual LED array relatively simple in structure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces the image collection time by a factor of three, allowing for the observation of dynamic sample activities while maintaining high spatial resolution, thus enhancing the system's applicability in biological imaging.
Implementation Method 1
a three-color LED array; a microscope, configured to obtain image information with multi-wavelength generated by illuminating a sample with the three-color LED array
Implementation Method 2
a microscope, configured to obtain image information with multi-wavelength generated by illuminating a sample with the three-color LED array, and to magnify the image information to generate magnified image information
Implementation Method 3
an RGB camera, disposed on an image plane of the microscope, and configured to acquire a first color image with a first resolution based on the magnified image information
Data Source
AI summary
The present disclosure relates to Fourier ptychographic microscopy systems and methods. The system includes: a three-color LED array; a microscope, configured to obtain image information with multi-wavelength, and magnify to generate magnified image information; an RGB camera configured to acquire a first color image with a first resolution based on the magnified image information; and a controller, configured to synchronously control the three-color LED array and the RGB camera, in which the three-color LED array is further configured to display a plurality of illumination patterns, the RGB camera is further configured to acquire synchronously a plurality of first color images, and the controller is further configured to restore a single second image with a second resolution according to the plurality of first color images, and the first resolution is less than the second resolution. The present disclosure improves sampling speed.