Wheat Variety 6PJRS71B Gene Editing for Trait Stability
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Solution Overview
Problem
Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved agronomic characteristics in a single wheat variety, while maintaining genetic stability and uniformity.
Innovation Solution
The development of the wheat variety 6PJRS71B, which involves genetic modification and locus conversion through transgenic techniques, including CRISPR/Cas system, to introduce traits like herbicide resistance, disease resistance, and improved nutritional quality, along with traditional breeding methods like backcrossing and selection to ensure stability and uniformity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If traditional breeding methods are used to combine multiple desirable traits, then the number of breeding steps and intervention complexity increase, but the ability to achieve stable and uniform trait combination decreases
Solution Approach 1:
The patent applies gene editing technology to directly modify specific genetic parameters (loci) to introduce desirable traits such as herbicide resistance, disease resistance, and nutritional quality improvements. This approach changes the breeding process from multi-generational phenotypic selection to targeted genomic modification, reducing process complexity while achieving multiple trait combinations in a single variety.
2Adaptability or versatility
If multiple traits are combined through extensive breeding intervention, then trait diversity improves, but genetic stability and uniformity of the final variety deteriorate
Solution Approach 1:
The patent segments the breeding process into targeted gene editing events at specific loci rather than whole-genome recombination through traditional crossing. Each desirable trait is introduced or modified at its specific genetic locus independently, allowing precise control over trait composition while maintaining the stability of the rest of the genome. This segmentation approach ensures genetic uniformity while achieving diverse trait combinations.
3Loss of time
If genetic modification techniques are used to introduce desirable traits, then breeding time and resource investment decrease, but the complexity of genetic manipulation increases
Solution Approach 1:
The patent replaces mechanical breeding methods (crossing, selection, and phenotypic screening across multiple generations) with molecular-level genetic manipulation using CRISPR/Cas systems and other gene editing technologies. This substitution enables direct modification of target loci without requiring extensive generational cycling, dramatically reducing breeding time despite the increased complexity of molecular techniques.
Data Source
AI summary
A wheat variety designated 6PJRS71B, the plants and seeds of wheat variety 6PJRS71B, methods for producing a wheat plant produced by crossing the variety 6PJRS71B with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 6PJRS71B with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety 6PJRS71B are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety 6PJRS71B and to wheat varieties or breeding lines produced by those methods are also provided.