Wheat Variety 6PKJH92B Breeding Segmentation

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Solution Overview

Problem

Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved milling properties into a single variety, while maintaining uniformity and stability across different growing conditions.

Innovation Solution

The development of the wheat variety 6PKJH92B, which involves genetic modification and locus conversion techniques, including transgenic approaches and backcrossing, to introduce traits like herbicide resistance, disease resistance, and improved nutritional quality, while ensuring homozygosity and phenotypic stability through rigorous breeding and selection methods.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If multiple desirable traits are combined into a single wheat variety through breeding, then the variety exhibits improved seed yield, disease resistance, and agronomic characteristics, but the complexity of the breeding process increases significantly

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The breeding process is segmented into distinct generations (P, F1, F2, F3, etc.) with specific selection objectives for each generation. This segmentation allows systematic accumulation of desirable traits while managing complexity at each stage rather than attempting to combine all traits simultaneously.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Parental lines are pre-selected and characterized for specific desirable traits before crossing. The P generation parents are specifically chosen to contribute particular traits (e.g., disease resistance from one parent, yield potential from another), allowing the breeding program to start with predetermined trait combinations rather than searching through random variations.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If rigorous breeding and selection methods are used to ensure homozygosity and phenotypic stability, then the wheat variety exhibits uniformity across growing conditions, but the time required for variety development increases

Engineering Contradiction:
Improvephenotypic stabilityVSAvoidvariety development time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The breeding program employs periodic cycles of crossing, selfing, and selection across multiple generations (F1, F2, F3, F4, etc.). Each generation represents a periodic cycle where heterozygosity is reduced and homozygosity is increased through systematic self-pollination and selection, ultimately achieving phenotypic stability in the F6 generation and later.

Inventive Principle:
Principle #19Periodic action

Solution Approach 2:

Phenotypic evaluation and selection are performed at each generation based on observed traits. This feedback mechanism allows breeders to identify and eliminate off-type plants, maintain desired trait combinations, and accelerate the fixation of homozygous genotypes. Molecular marker analysis provides additional feedback to track genetic composition and verify homozygosity at specific loci.

Inventive Principle:
Principle #23Feedback

3Manufacturing precision

If transgenic approaches and locus conversion techniques are used to introduce specific traits, then the precision of trait introduction is improved, but the complexity of genetic manipulation increases

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidgenetic manipulation complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

Molecular markers serve as intermediaries to track and verify the presence and homozygosity of target alleles during breeding. These markers are linked to desirable traits and allow breeders to select plants carrying the desired genetic modifications without directly observing the trait expression, thereby increasing precision in trait introduction while simplifying the selection process.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding program utilizes controlled changes in genetic parameters through backcrossing to recurrent parents. By repeatedly backcrossing F1 hybrids to the recurrent parent and selecting for the introgressed trait, the program achieves precise introduction of specific alleles while recovering the majority of the recurrent parent's genome, thereby controlling the extent of genetic change.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11696541B2Wheat variety 6PKJH92B
Publication Date: 2023.07.11 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated 6PKJH92B, the plants and seeds of wheat variety 6PKJH92B, methods for producing a wheat plant produced by crossing the variety 6PKJH92B with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 6PKJH92B with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety 6PKJH92B are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety 6PKJH92B and to wheat varieties or breeding lines produced by those methods are also provided.