Wheat Variety 6PVEJ41B CRISPR Trait Integration
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Solution Overview
Problem
Current wheat breeding techniques face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved agronomic characteristics into a single wheat variety, while maintaining uniformity and stability across generations.
Innovation Solution
The development of the wheat variety 6PVEJ41B, which involves genetic modification and locus conversion through transgenic techniques, including CRISPR/Cas system, to introduce traits like herbicide resistance, disease resistance, and improved nutritional quality, along with traditional breeding methods like backcrossing and selection to ensure stability and uniformity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional breeding methods are used to combine multiple desirable traits, then the complexity of the breeding process increases, but the uniformity and stability of trait expression across generations deteriorates
Solution Approach 1:
The patent replaces traditional mechanical breeding methods (crossing, selection, and recombination of multiple parental lines) with genetic engineering techniques (CRISPR/Cas9, transgenic insertion) to directly introduce desired traits. This substitution allows precise control over trait inheritance and ensures uniformity across generations by targeting specific genomic loci rather than relying on complex multi-generational selection processes.
2Manufacturing precision
If genetic modification techniques are used to introduce desired traits, then the precision of trait introduction improves, but the complexity of the genetic modification process increases
Solution Approach 1:
The patent segments the complex genetic modification process into distinct modules: (1) CRISPR/Cas9-based targeted genome editing for precise locus conversion, (2) transgenic insertion for introducing foreign genes, and (3) traditional backcrossing for trait stabilization. Each module can be independently optimized and executed, reducing overall process complexity while maintaining high precision in trait introduction.
3Adaptability or versatility
If multiple breeding steps with significant intervention are used, then the improvement in desirable traits increases, but the number of breeding steps and time required increases
Solution Approach 1:
The patent employs preliminary action by using CRISPR/Cas9 to pre-edit the genome at target loci before crossing, and by using marker-assisted selection to pre-identify plants with desired traits early in the breeding process. This eliminates the need for multiple generations of phenotypic selection and significantly reduces the number of breeding steps required to achieve stable trait expression.
Data Source
AI summary
A wheat variety designated 6PVEJ41B, the plants and seeds of wheat variety 6PVEJ41B, methods for producing a wheat plant produced by crossing the variety 6PVEJ41B with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 6PVEJ41B with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety 6PVEJ41B are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety 6PVEJ41B and to wheat varieties or breeding lines produced by those methods are also provided.