Wheat Variety A060263H1 Trait Stacking via Transgenic Preliminary Action

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Solution Overview

Problem

Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved agronomic characteristics in a single wheat variety, while maintaining uniformity and stability across generations.

Innovation Solution

The development of the wheat variety A060263H1, which involves genetic modification and locus conversion through transgenic techniques, including the introduction of specific genes for traits like herbicide resistance, disease resistance, and abiotic stress tolerance, along with traditional breeding methods like backcrossing and selection, to create a stable and uniform germplasm.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to combine multiple desirable traits, then the number of traits combined increases, but the time required for breeding and the complexity of the breeding program increase significantly

Engineering Contradiction:
Improvenumber of traits combinedVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-introducing multiple desirable traits through genetic modification before traditional breeding begins. Transgenic techniques are used to insert genes for herbicide resistance, disease resistance, and abiotic stress tolerance into the wheat genome in advance, so that these traits are already present when backcrossing and selection commence, thereby reducing the overall breeding time

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular markers as intermediaries to track and select for desirable traits during the breeding process. These markers serve as genetic tags that allow breeders to efficiently identify plants carrying the desired transgenic traits and parental genome segments, accelerating the selection process and reducing the time required to develop stable varieties

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If multiple traits are introduced through genetic modification, then the desired traits are achieved, but the complexity of the genetic modification process increases

Engineering Contradiction:
Improvedesired traits achievedVSAvoidgenetic modification process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the complex genetic modification process into separate, manageable steps. Each desirable trait (herbicide resistance, disease resistance, abiotic stress tolerance) is introduced through separate transgenic events or in a staged manner, allowing for individual optimization and selection of each trait before combining them in the breeding program

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent uses molecular markers and selective agents as intermediaries to simplify the management of multiple transgenic traits. These tools allow for easy identification and selection of plants carrying specific trait combinations, reducing the complexity of tracking and managing multiple genetic modifications throughout the breeding process

Inventive Principle:
Principle #24Intermediary (Mediator)

3Stability of the object's composition

If extensive backcrossing is performed to maintain parental genome, then the uniformity of the variety is improved, but the number of breeding generations and time required increase

Engineering Contradiction:
Improveuniformity of varietyVSAvoidbreeding generations
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent applies feedback by using molecular marker-assisted selection to monitor the genomic composition of breeding lines at each generation. This feedback mechanism allows breeders to make informed decisions about which plants to advance, ensuring rapid recovery of the parental genome and maintaining uniformity without requiring the full number of traditional backcrossing generations

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent uses preliminary action by pre-selecting plants with the desired level of parental genome recovery using molecular markers before proceeding to the next breeding generation. This preliminary screening prevents the advancement of lines that would require additional backcrossing, thereby reducing the total number of generations needed to achieve uniformity

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10028473B2Wheat variety A060263H1
Publication Date: 2018.07.24 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated A060263H1, the plants and seeds of wheat variety A060263H1, methods for producing a wheat plant produced by crossing the variety A060263H1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety A060263H1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety A060263H1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety A060263H1 and to wheat varieties or breeding lines produced by those methods are also provided.