Wheat Allergen-Binding Nucleic Acid Aptamer for Stable Detection
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Solution Overview
Problem
Current methods for detecting wheat allergens, such as using antibodies, face challenges due to the instability of antibodies, making it difficult to perform cost-effective and easy tests.
Innovation Solution
Development of a wheat allergen-binding nucleic acid molecule with a dissociation constant of 20 nM or less, which can bind to wheat allergens, allowing for accurate detection in samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If antibodies are used for detecting wheat allergens, then detection capability is achieved, but stability and cost-effectiveness deteriorate
Solution Approach 1:
The patent uses aptamers (nucleic acid molecules) as synthetic copies that mimic the binding function of antibodies. These aptamers are selected through SELEX (Systematic Evolution of Ligands by EXponential enrichment) to specifically bind wheat allergens, providing a stable alternative to protein-based antibodies while maintaining detection capability.
Solution Approach 2:
The nucleic acid-based aptamers replace expensive and unstable antibodies with cheaper, more stable molecular structures. Nucleic acids are inherently more stable than proteins under various conditions and can be synthesized at lower cost, making the detection method more cost-effective and easier to implement.
2Measurement precision
If antibodies are used for wheat allergen detection, then specific binding is achieved, but ease of operation and cost deteriorate
Solution Approach 1:
The patent replaces the complex protein-based antibody system with a simpler nucleic acid-based aptamer system. This substitution simplifies the overall test method, making it easier to operate while maintaining specific binding capability through the high-affinity interaction between aptamers and their target allergens.
3Reliability
If conventional detection methods are used, then wheat allergen detection is possible, but accuracy and reliability deteriorate due to antibody instability
Solution Approach 1:
The patent fundamentally changes the chemical parameter of the binding agent from protein-based antibodies to nucleic acid-based aptamers. This parameter change results in improved stability, reliability, and accuracy of wheat allergen detection, as nucleic acids are more resistant to degradation and maintain consistent binding properties under various storage and test conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The nucleic acid molecule enables high-accuracy detection of wheat allergens in food manufacturing, management, and distribution, providing a reliable alternative to antibody-based methods.
Implementation Method 1
a wheat allergen-binding nucleic acid molecule that binds to a wheat allergen with a dissociation constant of 20 nM or less
Data Source
AI summary
The present invention provides a novel nucleic acid molecule that can be used for detection of a wheat allergen. The wheat allergen-binding nucleic acid according to the present invention is characterized in that it binds to a wheat allergen with a dissociation constant of 20 nM or less, and preferably includes a polynucleotide consisting of either of base sequences of SEQ ID NOs: 1 and 2, for example.