Wheat Variety Stabilization Through Backcrossing and Trait Stacking

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Solution Overview

Problem

Existing wheat varieties lack a comprehensive combination of desirable traits such as higher seed yield, disease resistance, drought and heat tolerance, altered milling properties, and improved agronomic characteristics, limiting their adaptability and commercial utility.

Innovation Solution

Development of the wheat variety 6PLWJ20B, which is homozygous and stable, incorporating genetic modifications and locus conversions to enhance traits like herbicide resistance, disease resistance, and abiotic stress tolerance, achieved through methods like genetic transformation and backcrossing, enabling the introduction of specific traits such as altered starch and nutritional quality.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional wheat breeding methods are used, then existing varieties are maintained, but they lack comprehensive combination of desirable traits such as higher seed yield, disease resistance, and drought tolerance

Engineering Contradiction:
ImproveadaptabilityVSAvoidtrait combination consistency
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct stages: initial cross-breeding to combine multiple parental traits, followed by systematic backcrossing to stabilize specific desirable traits while eliminating unwanted variations. This segmentation allows for controlled integration of seed yield, disease resistance, and drought tolerance traits from different parental lines.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Multiple generations of preliminary breeding and selection are performed before final variety stabilization. The process includes preliminary cross-breeding to establish trait combinations, followed by preliminary backcrossing generations to progressively refine and stabilize the desired trait profile before commercial deployment.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If genetic modifications are introduced to enhance traits like herbicide resistance and disease resistance, then the wheat variety gains improved resistance, but the complexity of development increases

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

Traditional backcrossing serves as an intermediary mechanism to bridge the introduction of genetic modifications and the final stable variety. The systematic backcrossing process acts as a mediator that gradually integrates resistance traits while managing developmental complexity through controlled, incremental generations.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding process utilizes parameter changes across multiple generations, systematically altering genetic composition through controlled crossing and selection. Each backcrossing generation represents a parameter change that progressively refines the trait profile, managing complexity through controlled temporal evolution rather than simultaneous modification.

Inventive Principle:
Principle #35Parameter changes

3Stability of the object's composition

If multiple backcrossing generations are performed to stabilize traits, then trait stability is achieved, but the time required for variety development increases

Engineering Contradiction:
Improvevariety stabilityVSAvoiddevelopment time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

Preliminary cross-breeding and trait selection are performed in advance to establish the foundation for stability before entering the backcrossing phase. This preliminary action prepares the genetic material in advance, allowing the subsequent backcrossing generations to focus specifically on stabilizing traits rather than simultaneously creating and stabilizing them.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The backcrossing process maintains continuous useful action through systematic, uninterrupted generations of crossing and selection. Each generation builds upon the previous one without interruption, continuously progressing toward trait stability. This continuity prevents loss of momentum and ensures steady accumulation of stabilizing effects across generations.

Inventive Principle:
Principle #20Continuity of useful action

Data Source

PatentUS12484503B2Wheat variety 6PLWJ20B
Publication Date: 2025.12.02 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated 6PLWJ20B, the plants and seeds of wheat variety 6PLWJ20B, methods for producing a wheat plant produced by crossing the variety 6PLWJ20B with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety 6PLWJ20B with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety 6PLWJ20B are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety 6PLWJ20B and to wheat varieties or breeding lines produced by those methods are also provided.