Indoor Wheat Black Point Resistance Identification Method
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for identifying wheat resistance to black point disease caused by Bipolaris sorokiniana are time-consuming, labor-intensive, and yield unstable results, limiting the progress of disease resistance breeding and mechanism research due to reliance on field-based identification processes that are seasonally restricted and resource-intensive.
Innovation Solution
A method for rapidly identifying wheat resistance by calculating black point incidence through the percentage of diseased leaf area caused by Bipolaris sorokiniana at the seedling stage in an indoor controlled environment, using surface sterilization, seedling cultivation, inoculation with conidial suspensions, and regression analysis to evaluate resistance levels.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If field-based identification methods are used to assess wheat resistance to black point disease, then the evaluation reflects real-world conditions, but the process is time-consuming, labor-intensive, and seasonally restricted
Solution Approach 1:
The patent applies preliminary action by conducting pathogen inoculation and disease development assessment during the seedling stage (3-4 leaf stage) rather than waiting for the grain filling stage. This allows resistance evaluation to be performed early in the wheat growth cycle, enabling multiple assessments per season and accelerating breeding programs while maintaining evaluation reliability through controlled inoculation procedures
Solution Approach 2:
The patent segments the identification process into controlled indoor components: pathogen culture preparation, seedling inoculation, disease symptom observation, and resistance classification. This segmentation allows the evaluation to be conducted in controlled environmental conditions during the seedling stage, decoupling it from seasonal field constraints while preserving evaluation accuracy through standardized procedures
2Reliability
If field-based identification methods are used, then natural disease occurrence is observed, but the process requires substantial manpower and material resources
Solution Approach 1:
The patent prepares pathogen inoculum in advance through controlled culture procedures and pre-establishes standardized evaluation criteria. This preliminary preparation eliminates the need for extensive field resources during actual assessment, as the pathogen and evaluation framework are ready beforehand, significantly improving resource efficiency while maintaining evaluation stability
Solution Approach 2:
The patent creates a controlled indoor replication of field disease conditions by inoculating seedlings with standardized pathogen concentrations in controlled environments. This copying approach allows evaluation without extensive field resources, as the essential disease interaction is replicated in a resource-efficient laboratory setting while maintaining evaluation reliability through standardized protocols
3Measurement precision
If identification is conducted only during grain filling stage, then the assessment reflects actual black point disease occurrence, but it can only be performed once per growth season
Solution Approach 1:
The patent performs preliminary inoculation and assessment during the seedling stage, allowing resistance evaluation to occur before the grain filling stage. This preliminary assessment provides an early indication of resistance that correlates with grain filling stage outcomes, enabling multiple evaluations per season and improving identification frequency while maintaining measurement precision through validated correlation
Solution Approach 2:
The patent shifts the evaluation from the traditional grain filling stage to the seedling stage, adding a temporal dimension to the identification process. This dimensional change allows assessments to be conducted earlier in the growth cycle when environmental constraints are minimal, enabling multiple time points per season while maintaining accuracy through correlation with grain filling stage resistance
Data Source
AI summary
The present application discloses a method for rapidly identifying the resistance of wheat to black point disease caused by Bipolaris sorokiniana, where testing takes places in an indoor, controlled environment. The test method includes surface sterilization of wheat seeds, and cultivating wheat seedlings from the sterilized wheat seeds; preparing conidial suspension of Bipolaris sorokiniana, and spraying the conidial suspension on the seedlings at one-leaf-one-shoot stage; recording the percentage of the diseased leaf area in total leaf area of the first leaf of the wheat seedling on the 10th day of inoculation; calculating the black point incidence of the wheat according to an equation, and then evaluating the resistance of wheat to black point disease caused by Bipolaris sorokiniana through the black point incidence. Compared with existing field identification methods, the method of the present disclosure shortens identification time, simplifies identification procedure, greatly improves identification efficiency, and improves the accuracy and reliability of identification results.