Wheat Embryo Isolation by Single-Impact Separation for High Purity
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Solution Overview
Problem
Current methods for isolating wheat embryos from wheat berries result in contamination with endosperm particles and tritin, leading to low purity and viability issues, hindering their use in industrial-scale cell-free protein synthesis.
Innovation Solution
A method involving controlled single-impact acceleration and orientation of wheat berries against a smooth impact surface, followed by separation techniques such as screening and floatation, to produce highly pure and viable wheat embryos.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional methods are used to separate wheat embryos from wheat berries, then wheat germ can be produced, but the wheat germ is contaminated with endosperm particles and tritin, resulting in low purity
Solution Approach 1:
The invention segments the wheat berry into distinct components (embryo, endosperm, bran) through controlled mechanical action. The embryo is separated from the endosperm and bran through careful mechanical disruption that preserves embryo integrity while removing contaminants, achieving high purity wheat germ production
Solution Approach 2:
The invention extracts the embryo from the wheat berry structure through controlled mechanical action. By applying specific mechanical forces, the embryo is extracted from the endosperm and bran matrix, allowing for pure wheat germ isolation without contamination from tritin-containing endosperm particles
2Quantity of substance
If wheat berries are crushed to extract embryos, then embryos can be released, but the embryos are killed and decomposition products are generated
Solution Approach 1:
The invention applies beforehand cushioning by using controlled mechanical action that prevents excessive force on the embryos. The mechanical process is designed to disrupt the wheat berry structure sufficiently to release embryos while cushioning against forces that would kill the embryos or generate decomposition products
Solution Approach 2:
The invention changes the mechanical parameters of the extraction process to preserve embryo viability. By controlling the type, intensity, and duration of mechanical action, the process extracts embryos without subjecting them to lethal forces or conditions that would initiate decomposition
3Manufacturing precision
If repeated beating is applied to separate wheat embryos, then separation improves, but the embryos suffer fissures, chips and breaks that are lethal
Solution Approach 1:
The invention uses periodic action through controlled mechanical cycles that gradually separate embryos from the wheat berry matrix. Rather than repeated violent beating, the process employs periodic mechanical action that achieves separation through cumulative effect while allowing embryos to survive each cycle intact
Solution Approach 2:
The invention applies beforehand cushioning by designing the mechanical process to prevent excessive forces on embryos. The controlled mechanical action cushions against the generation of fissures, chips, and breaks that would be lethal to embryos, while still achieving effective separation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Achieves wheat embryos with over 99.9% purity and high viability, suitable for industrial-scale cell-free protein synthesis, avoiding tritin contamination and decomposition.
Implementation Method 1
accelerating a plurality of wheat berries toward an impact surface, impacting each of the plurality of wheat berries against the impact surface, dislodging at least some of the wheat embryos from the wheat berries in response to the impacting step
Implementation Method 2
separating the dislodged wheat embryos from the bran and the endosperm to produce an intermediate purified wheat embryo product
Data Source
AI summary
Methods for producing a purified wheat embryo product are disclosed. In one embodiment, producing a purified wheat embryo product includes the steps of: accelerating a plurality of wheat berries toward an impact surface, impacting each of the plurality of wheat berries against the impact surface, dislodging at least some of the wheat embryos from the wheat berries in response to the impacting step such that the dislodged embryos are intact, and separating the dislodged wheat embryos from the bran and the endosperm to produce an intermediate purified wheat embryo product.