Wheat Cultivar HV9W10-1002 Marker-Assisted Trait Stacking

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Solution Overview

Problem

Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, and drought tolerance into a single wheat cultivar effectively, while also maintaining stability across generations and environments.

Innovation Solution

The development of the wheat cultivar HV9W10-1002, which is a hard red winter type bred for fall planting, incorporating a single locus conversion that can introduce transgenic genes for traits like herbicide resistance, disease resistance, and improved nutritional quality, using genetic transformation and traditional breeding techniques, along with advanced plant breeding methods like backcrossing and marker-assisted selection.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to combine multiple desirable traits, then the complexity of the breeding program increases, but the ability to effectively combine and stabilize traits across generations decreases

Engineering Contradiction:
Improveability to combine desirable traitsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent segments the breeding process by using molecular markers to identify and track specific trait-associated DNA sequences independently. This allows breeders to select for multiple traits simultaneously through marker-assisted selection rather than relying on complex phenotypic evaluation of all traits together, thereby simplifying the overall breeding program while effectively combining multiple desirable traits.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent introduces molecular markers as intermediaries between the desired traits and the selection process. These markers serve as genetic tags that indirectly indicate the presence of desirable trait alleles, enabling breeders to select plants with multiple desired traits more efficiently without directly evaluating each trait's phenotypic expression, thus reducing breeding program complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If multiple traits are combined in a single cultivar through conventional breeding, then the number of breeding generations required increases, but the stability of trait expression across environments decreases

Engineering Contradiction:
Improvetrait stability across environmentsVSAvoidbreeding duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using molecular markers to identify and select plants carrying desired trait alleles early in the breeding process, before phenotypic expression and environmental variability can affect trait stability. This allows breeders to fix desirable traits in the genetic makeup of the cultivar through selective breeding based on marker data, ensuring stable trait expression across environments while reducing the number of generations needed compared to conventional phenotypic selection methods.

Inventive Principle:
Principle #10Preliminary action

3Manufacturing precision

If genetic transformation is used to introduce specific traits, then the precision of trait introduction improves, but the regulatory and public acceptance challenges increase

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidregulatory complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent employs copying by using molecular markers that replicate the information about desired trait alleles without requiring actual genetic transformation. Instead of introducing foreign DNA through transformation, the method copies and tracks existing favorable alleles through marker-assisted selection, achieving precise trait combination while avoiding the regulatory and public acceptance issues associated with transgenic modification.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS9826692B2Wheat cultivar HV9W10-1002
Publication Date: 2017.11.28 MONSANTO TECHNOLOGY LLC

AI summary

The invention relates to the wheat cultivar designated HV9W10-1002. Provided by the invention are the seeds, plants and derivatives of the wheat cultivar HV9W10-1002. Also provided by the invention are tissue cultures of the wheat cultivar HV9W10-1002 and the plants regenerated therefrom. Still further provided by the invention are methods for producing wheat plants by crossing the wheat cultivar HV9W10-1002 with itself or another wheat cultivar and plants produced by such methods.