Wheat Event IND-ØØ412-7 Abiotic Stress Tolerance
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Solution Overview
Problem
Current methods for generating commercially suitable transgenic events in crops like wheat are labor-intensive and require extensive characterization to ensure desired traits such as drought tolerance are expressed without affecting other agricultural features.
Innovation Solution
The development of wheat transgenic event IND-ØØ412-7, which incorporates the HaHB4 gene from sunflower, providing tolerance to abiotic stresses like drought, salinity, and temperature fluctuations, along with the bar gene for herbicide resistance, using specific promoters and terminators for regulated expression.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic events are generated to improve crop tolerance to abiotic stresses, then the tolerance is improved, but the process requires extensive characterization and analysis making it labor-intensive and time-consuming
Solution Approach 1:
The patent applies preliminary action by pre-characterizing multiple transgenic events in the laboratory under controlled conditions before field testing. This includes preliminary molecular characterization to identify events with desired traits, reducing the time and labor required during commercial deployment
Solution Approach 2:
The patent segments the selection process into distinct stages: laboratory development with molecular characterization, controlled environment testing, and field trials. This segmentation allows systematic evaluation and reduces overall time by parallel processing different characterization aspects
2Manufacturing precision
If extensive characterization and analysis are performed to select suitable transgenic events, then the desired traits are ensured, but the process complexity increases
Solution Approach 1:
The characterization process is divided into modular stages: molecular characterization, phenotypic analysis under controlled conditions, and field performance evaluation. Each stage uses specific standardized protocols, reducing overall process complexity while maintaining selection precision
Solution Approach 2:
The patent changes evaluation parameters systematically across different stages - using molecular markers in laboratory, controlled environmental parameters in greenhouses, and field conditions for final selection. This parameter progression simplifies the complex selection process by breaking it into manageable steps
3Reliability
If multiple transgenic events are analyzed and tested under various environmental conditions, then the suitable event is identified, but the resource consumption increases
Solution Approach 1:
Laboratory-based molecular and phenotypic characterization is performed as a preliminary filter to identify promising transgenic events before allocating resources for extensive field trials. This preliminary screening reduces resource consumption by eliminating unsuitable events early in the process
Solution Approach 2:
The patent performs characterization under a variety of environmental conditions exceeding minimum requirements to ensure robust event selection. This excessive action in controlled laboratory settings prevents the need for repeated field testing, ultimately reducing overall resource consumption
Data Source
AI summary
The present invention refers to a wheat plant, seed or part of it comprising event IND-ØØ412-7, a commodity product resulting from the seed, a recombinant DNA molecule comprised in event IND-ØØ412-7, DNA primers and probes useful for detecting event IND-ØØ412-7, DNA detection kit, method for producing a wheat plant tolerant to abiotic stresses and method for detecting the presence of DNA belonging to wheat event IND-ØØ412-7 in a sample.