Wheat MS45 Promoters for Hexaploid Male Sterility Control
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Solution Overview
Problem
Hexaploidy in wheat has hindered the understanding of how homologous genes interact, their expression is regulated, and how the different proteins function, posing a significant obstacle in developing useful variants, particularly in influencing male fertility and promoters associated with such genes.
Innovation Solution
Compositions and methods involving nucleotide sequences, fragments, and variants that modulate male fertility in plants, including expression cassettes with operably linked promoters to influence male fertility, utilizing wheat MS45 promoter sequences and related polynucleotides to restore or manipulate male fertility.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If hexaploid wheat is used for breeding, then crop yield and desirable characteristics are improved, but understanding of homologous gene interaction and regulation is hindered
Solution Approach 1:
The patent segments the complex hexaploid wheat genome by focusing on specific promoter regions (MS45 promoter) and their homologous sequences across different genomes (A, B, and D genomes). This segmentation allows researchers to study individual promoter elements and their functional variants without being overwhelmed by the entire hexaploid genome complexity, while still benefiting from the overall genetic diversity of hexaploid wheat for breeding purposes.
2Productivity
If male sterility systems are manipulated for hybrid breeding, then hybridization efficiency is improved, but control over male fertility becomes difficult
Solution Approach 1:
The patent utilizes parameter changes by identifying and manipulating specific promoter sequences (MS45 promoter) and their variants that control male fertility. By changing the presence, absence, or variation of these promoter elements, the system can precisely control male fertility states (fertile, sterile, or partially fertile) in a predictable manner, enabling efficient hybrid breeding while maintaining operational control through molecular markers and targeted genetic modifications.
Data Source
AI summary
Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid.