Wheat Variety W010098F2 Breeding via Molecular Markers

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Solution Overview

Problem

Current wheat breeding methods face challenges in developing stable, high-yielding wheat varieties with improved grain quality and resistance to diseases and environmental stresses, requiring innovative approaches to enhance genetic diversity and trait introduction.

Innovation Solution

The development of the wheat variety W010098F2 through a modified pedigree selection method, combined with advanced breeding techniques such as backcross conversion and transformation, to introduce desirable traits like disease resistance, drought tolerance, and improved grain quality, utilizing molecular markers and genetic engineering to enhance agronomic characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional wheat breeding methods are used, then existing varieties can be maintained, but genetic diversity and resistance to diseases and environmental stresses are limited

Engineering Contradiction:
Improveresistance to diseases and environmental stressesVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent employs a donor parent line with superior disease resistance and stress tolerance traits that is crossed with the recurrent parent. This intermediary donor parent introduces desirable genes (e.g., rust resistance, drought tolerance) into the breeding program, resolving the contradiction by providing a source of genetic diversity without requiring complex multi-parent crosses or extensive mutagenesis screens.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding program utilizes molecular markers and genomic selection to change the parameters of trait identification and selection. By using DNA-based markers linked to disease resistance genes and stress tolerance traits, the program can screen large populations efficiently, reducing the complexity of traditional phenotypic screening while achieving superior adaptability.

Inventive Principle:
Principle #35Parameter changes

2Reliability

If advanced breeding techniques like backcross conversion and transformation are used, then desirable traits can be introduced, but the breeding process becomes more complex

Engineering Contradiction:
Improvestability of wheat varietyVSAvoidbreeding methodology complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent performs preliminary actions by creating a donor parent line with stable, superior traits through controlled crosses before initiating the main breeding program. The donor parent is developed and characterized in advance, so that when it is crossed with the recurrent parent, the desirable traits are already established, reducing the complexity of subsequent selection and stabilization steps.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces traditional mechanical breeding methods (repeated manual crossing and phenotypic screening) with molecular-based techniques. Genomic selection, molecular markers, and transformation methods are used to identify and stabilize desirable traits, reducing the number of generations required and simplifying the breeding methodology while achieving stable, reliable varieties.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Stability of the object's composition

If multiple breeding generations are performed to achieve homozygosity, then variety stability is improved, but time consumption increases

Engineering Contradiction:
Improvehomozygosity and variety stabilityVSAvoidbreeding program duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent substitutes traditional generational selfing and phenotypic screening with molecular marker-assisted selection and genomic selection. By using DNA markers linked to homozygous loci and genomic profiles, the program can identify and select stable, homozygous lines much faster than through conventional methods, reducing breeding program duration while maintaining variety stability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the parameter of stability assessment from phenotypic observation across multiple generations to molecular genetic analysis. By using molecular markers to directly assess homozygosity and genetic stability, the program can evaluate variety stability in a single generation, dramatically reducing time consumption while achieving the same level of homozygosity and stability.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS8598440B1Wheat variety W010098F2
Publication Date: 2013.12.03 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated W010098F2, the plants and seeds of wheat variety W010098F2, methods for producing a wheat plant produced by crossing the variety W010098F2 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W010098F2 with another wheat line or plant, and the creation of variants by mutagenesis or transformation of variety W010098F2. This invention also relates to methods for producing other wheat varieties or breeding lines derived from wheat variety W010098F2 and to wheat varieties or breeding lines produced by those methods.