Wheat Variety W070303B1 Breeding Segmentation

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Solution Overview

Problem

Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved agronomic characteristics in a single wheat variety, while maintaining uniformity and stability across generations.

Innovation Solution

The development of the wheat variety W070303B1, which involves genetic modification and locus conversion techniques, including transgenic approaches and backcrossing, to introduce traits like herbicide resistance, disease resistance, and improved nutritional quality, while ensuring homozygosity and phenotypic stability through rigorous breeding and selection methods.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If multiple desirable traits are combined in a single wheat variety through breeding, then the agronomic performance and resistance traits are improved, but the breeding process complexity and time required increase significantly

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The breeding process is segmented into distinct phases: initial cross-breeding to combine desired traits, followed by separate backcrossing generations (BC1, BC2, BC3) to recover the recurrent parent genome, and final selection. This segmentation allows systematic management of complex breeding operations while tracking specific resistance traits through each generation.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Molecular marker analysis is performed preliminarily at each breeding stage to identify and select plants carrying desired resistance genes before phenotypic expression occurs. This preliminary genetic screening enables early selection of promising lines, reducing the need to carry forward all phenotypic candidates through subsequent generations.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If rigorous selection methods are applied to ensure homozygosity and phenotypic stability, then the uniformity of the wheat variety is improved, but the time and resources required for breeding increase

Engineering Contradiction:
Improvephenotypic stabilityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

Molecular marker data provides continuous feedback on the genetic composition of breeding lines at each generation. This feedback mechanism allows breeders to objectively assess homozygosity levels and make informed decisions about which lines to advance, replacing subjective phenotypic selection with objective genetic information that accelerates the path to stability.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

Traditional mechanical/visual selection methods are replaced with molecular marker-based genetic analysis. This substitution enables detection of homozygosity and desired traits at the DNA level, eliminating the need for lengthy field testing and multiple generations of phenotypic observation to confirm genetic stability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Adaptability or versatility

If backcrossing is used to introgress specific traits while maintaining the recurrent parent genome, then the desired trait incorporation is improved, but the number of breeding generations and complexity increase

Engineering Contradiction:
Improvetrait incorporationVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

Molecular markers serve as intermediaries to track and identify the presence of desired resistance genes during backcrossing. These markers act as genetic signposts that enable selective breeding of plants carrying the target traits while recovering the recurrent parent genome, simplifying the management of complex backcrossing operations by providing clear selection criteria.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The selection criteria parameter is changed from phenotypic observation to molecular marker genotype analysis. This parameter change enables precise identification of plants carrying desired resistance genes at each backcross generation, allowing efficient trait incorporation while systematically recovering the recurrent parent genome with fewer generations than traditional phenotypic selection would require.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS10624294B2Wheat variety W070303B1
Publication Date: 2020.04.21 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated W070303B1, the plants and seeds of wheat variety W070303B1, methods for producing a wheat plant produced by crossing the variety W070303B1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W070303B1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety W070303B1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety W070303B1 and to wheat varieties or breeding lines produced by those methods are also provided.