Wheat Variety W090428N1 Breeding via Locus Conversion

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Solution Overview

Problem

Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved milling properties into a single variety, while maintaining uniformity and stability across generations.

Innovation Solution

The development of the wheat variety W090428N1, which involves genetic modification and locus conversion through transgenic techniques, including the introduction of specific traits like herbicide resistance, disease resistance, and altered nutritional content, combined with traditional breeding methods like backcrossing and selection, to create a stable and uniform wheat germplasm.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional breeding methods are used to combine desirable traits, then the variety development process is simple and maintains natural genetic diversity, but it requires numerous steps involving significant intervention and takes a long time to achieve stable combination of traits

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidbreeding cycle duration
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by performing genetic modification and locus conversion early in the breeding process, before traditional breeding steps. Transgenic techniques and locus conversion are used to introduce and stabilize desirable traits (disease resistance, drought tolerance, improved milling properties) in the parental lines before crossing, thereby reducing the number of subsequent breeding steps and accelerating the overall breeding cycle while maintaining genetic diversity

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If multiple desirable traits are combined into a single variety, then the agricultural performance and nutritional quality are improved, but the complexity of the breeding program and genetic modification processes increases

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the breeding program into distinct modular components: (1) genetic modification of parental lines to introduce specific traits (disease resistance, drought tolerance), (2) locus conversion to stabilize traits, (3) controlled crossing to combine traits, and (4) selection to achieve uniformity. This modular approach allows systematic combination of multiple desirable traits while managing complexity through structured organization

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent uses locus conversion as an intermediary mechanism to bridge genetic modification and traditional breeding. Locus conversion stabilizes transgenic traits and facilitates their integration into the breeding program, acting as a mediator between the complexity of genetic modification and the need for stable, uniform varieties with multiple desirable traits

Inventive Principle:
Principle #24Intermediary (Mediator)

3Reliability

If genetic modification and transgenic techniques are used, then specific traits like herbicide resistance and disease resistance are introduced efficiently, but the regulatory and scientific complexity increases

Engineering Contradiction:
Improvetrait stabilityVSAvoidgenetic modification complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent employs locus conversion as an intermediary process that stabilizes transgenic traits introduced through genetic modification. This intermediary step ensures trait reliability and uniformity across generations while managing the complexity of genetic modification by creating a stable platform for subsequent breeding operations

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent applies parameter changes by modifying specific genetic loci through locus conversion to achieve stable trait expression. By changing the genetic parameters at specific loci (through transgenic introduction and subsequent conversion), the patent achieves reliable trait stability while managing the complexity of genetic modification through targeted rather than genome-wide approaches

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS10709093B2Wheat variety W090428N1
Publication Date: 2020.07.14 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated W090428N1, the plants and seeds of wheat variety W090428N1, methods for producing a wheat plant produced by crossing the variety W090428N1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W090428N1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety W090428N1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety W090428N1 and to wheat varieties or breeding lines produced by those methods are also provided.