Wheat Variety W090489Z1 Breeding via Locus Conversion

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Solution Overview

Problem

Current wheat breeding methods face challenges in combining desirable traits such as high seed yield, disease resistance, drought tolerance, and improved agronomic characteristics into a single wheat variety, while maintaining uniformity and stability across generations.

Innovation Solution

The development of the wheat variety W090489Z1, which involves genetic modification and locus conversion through transgenic techniques, including the introduction of specific genes for traits like herbicide resistance, disease resistance, and altered nutritional content, combined with traditional breeding methods like backcrossing and selection, to create a stable and uniform germplasm.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional breeding methods are used to combine desirable traits, then genetic diversity is maintained, but the time required to develop a new variety is excessive and uniformity across generations cannot be guaranteed

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidtime to develop new variety
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by performing genetic modification and locus conversion before traditional breeding steps. Specific genes are introduced and converted to desired loci in advance, allowing subsequent breeding to focus only on combining these pre-modified lines, thereby significantly reducing the overall breeding time while maintaining uniformity through controlled genetic changes.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent utilizes parameter changes by altering the genetic parameter at specific loci through locus conversion. By changing the genetic state at particular locations in the genome rather than relying on random mutation and selection, the breeding process achieves desired trait combinations much faster while ensuring uniformity across generations through precise genetic modification.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If multiple genes are introduced through transgenic techniques, then desirable traits are achieved, but the complexity of the breeding program increases

Engineering Contradiction:
Improvetrait combination capabilityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the complex trait combination into separate modules - each gene or locus conversion is introduced and stabilized in separate lines before final combination. This modular approach allows the complex breeding program to be managed through discrete, controllable steps rather than attempting to introduce and combine all genes simultaneously, reducing overall program complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent uses intermediary lines as mediators between the different genetic modifications. By creating intermediate lines that each carry specific locus conversions or gene introductions and stabilize them before combining with other lines, the patent simplifies the management of multiple genes, allowing each modification to be optimized independently before integration.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If locus conversion is performed to introduce specific traits, then genetic precision is improved, but the risk of unintended genetic changes increases

Engineering Contradiction:
Improvegenetic modification precisionVSAvoidgenetic stability
Core Design Contradiction:
Manufacturing precisionVSReliability

Solution Approach 1:

The patent implements feedback mechanisms through rigorous testing and evaluation at multiple stages of locus conversion and line development. Genetic and phenotypic characteristics are continuously monitored and assessed, allowing for verification that the intended locus conversions have been achieved without unintended changes, thereby maintaining reliability while preserving precision.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent applies beforehand cushioning by performing multiple rounds of backcrossing and selection before final line establishment, creating a buffer against potential unintended genetic changes. This repetitive process ensures that any stray genetic variations are eliminated early, protecting the final genetic stability while maintaining the precision of the intended locus conversions.

Inventive Principle:
Principle #11Beforehand cushioning (Prior cushioning)

Data Source

PatentUS10757884B2Wheat variety W090489Z1
Publication Date: 2020.09.01 PIONEER HI BREED INTERNATIONAL INC

AI summary

A wheat variety designated W090489Z1, the plants and seeds of wheat variety W090489Z1, methods for producing a wheat plant produced by crossing the variety W090489Z1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety W090489Z1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety W090489Z1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety W090489Z1 and to wheat varieties or breeding lines produced by those methods are also provided.