Wound In-Situ Printing Device for Layered Tissue Repair
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Solution Overview
Problem
Current 3D printing technologies for skin defect repair face challenges such as the need for new capillary growth, difficulty in identifying skin surface orientation, high costs due to in vitro culture requirements, and inability to effectively treat wounds with bone, muscle, or fascia defects, leading to reduced survival rates and increased surgical time.
Innovation Solution
A wound in-situ printing device that directly prints bio-inks containing skin dermis, epidermis, bone, muscle, and fascia layers onto the wound, utilizing naturally formed capillaries for nutrition and eliminating the need for cross-linking agents, while providing a biomimetic dressing for real-time observation and reducing the need for expensive culture media and harsh incubation conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If 3D printed skin is cultured in vitro for a period of time, then the skin structure can be formed, but the cost of culture medium and maintaining the incubation environment becomes too expensive
Solution Approach 1:
The patent enables the skin scaffold to self-assemble and differentiate in situ within the wound bed without requiring external in vitro culture. The bio-ink containing skin precursor cells is printed directly onto the wound, and the body's natural environment provides the necessary conditions for tissue formation, eliminating the need for expensive culture media and incubation equipment.
Solution Approach 2:
The patent extracts the skin formation process from the external laboratory environment and relocates it to the in situ wound environment. By removing the requirement for in vitro culture, the system eliminates dependence on expensive culture media while maintaining the ability to form functional skin structures.
2Stability of the object's composition
If cross-linking measures are used to connect skin layers, then structural stability is improved, but the complexity of the repair process increases
Solution Approach 1:
The patent employs the body's natural wound healing processes to connect and stabilize skin layers without requiring external cross-linking agents or measures. The printed skin scaffold integrates with surrounding tissue through natural cellular migration, proliferation, and extracellular matrix deposition, simplifying the overall repair process.
3Adaptability or versatility
If the 3D printed skin is fragmented, then adaptability to wound shapes is improved, but the ability to accurately identify skin surface becomes difficult
Solution Approach 1:
The patent incorporates visual markers or contrasting materials within the bio-ink or skin scaffold structure that enable easy identification of the skin surface orientation. These visual indicators allow surgeons to quickly distinguish the correct orientation of fragmented skin pieces during implantation without requiring complex orientation markers.
4Ease of manufacture
If capillary network is lacking in dermis, then the printing process is simplified, but the survival rate of transplanted skin is reduced
Solution Approach 1:
The patent pre-forms a three-dimensional porous scaffold structure within the bio-ink that anticipates and facilitates subsequent capillary ingrowth. The scaffold's porous architecture is designed beforehand to guide blood vessel formation and nutrient delivery, ensuring high survival rates without requiring complex pre-vascularization procedures.
Solution Approach 2:
The patent utilizes a porous scaffold material with optimized pore size and distribution that promotes rapid capillary infiltration and blood vessel formation. The porous structure allows easy passage of cells and nutrients while maintaining mechanical integrity, solving the contradiction between printing simplicity and survival rate.
Data Source
AI summary
A method of a wound in-situ printing repair includes spraying a bio ink in stages and layers on the wound surface with a wound in-situ printing device, and providing an incubation microenvironment. The wound surface can be layered with newly formed bone, muscle, subcutaneous fat, appendage, dermis and epidermis, so as to achieve a physiological repair of the wound. The wound in-situ printing device includes a filling component, a container component, a spray printing component, and a control component to form an amniotic cavity like biomimetic structure in the wound surface, and intelligently implement tissue bioprinting.