Maize Hybrid X11T099 with CMS for Uniform Trait Introgression
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Solution Overview
Problem
Existing hybrid maize development methods struggle to combine desirable traits such as disease resistance, heat and drought tolerance, and improved yield while maintaining uniformity and stability in commercial crops.
Innovation Solution
A novel maize hybrid variety X11T099 is developed by crossing inbred varieties and introducing genetic loci for traits like male sterility, disease resistance, and herbicide tolerance through backcrossing and transformation, with cytoplasmically-inherited traits ensuring trait inheritance and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional plant breeding methods are used to combine multiple desirable traits, then the complexity of the breeding program increases, but the uniformity and stability of the hybrid decreases
Solution Approach 1:
The patent segments the breeding process by using separate inbred parent lines (PH47GA and 1PCDF91) that each carry specific desirable traits. By maintaining distinct parental lines with homozygous genotypes and crossing them to produce F1 hybrids, the method combines multiple traits (disease resistance, drought tolerance, yield improvement) while ensuring uniformity in the hybrid generation through controlled segmentation of genetic contributions from each parent.
Solution Approach 2:
The patent applies preliminary action by developing and stabilizing inbred parent lines through multiple generations of selfing and selection before the actual hybridization. The parental lines are pre-conditioned to be homozygous and genetically stable, which ensures that when crossed, they produce uniform F1 hybrids with consistent expression of desirable traits, thereby maintaining stability while combining multiple characteristics.
2Reliability
If multiple traits are introduced through backcrossing and transformation, then the agronomic quality improves, but the time required for variety development increases
Solution Approach 1:
The patent uses copying by creating true-breeding inbred parent lines that can be repeatedly crossed to produce identical F1 hybrid generations. The parental lines serve as stable genetic templates that can be propagated and used across multiple breeding cycles, reducing the time required to redevelop traits. The consistent copying of parental genotypes ensures reliable agronomic quality without extending development time across generations.
Solution Approach 2:
The patent applies parameter changes by utilizing cytoplasmic male sterility (CMS) as a controllable parameter to facilitate hybrid seed production. By introducing CMS into the breeding system, the patent eliminates the need for manual detasseling, accelerates hybrid seed production, and reduces development time while maintaining agronomic quality. The CMS parameter allows for efficient production of uniform hybrids with desired traits.
3Productivity
If cytoplasmic male sterility is introduced to prevent self-pollination, then hybrid seed production efficiency improves, but the device complexity increases
Solution Approach 1:
The patent applies self-service by using cytoplasmic male sterility to enable the female parent to automatically prevent self-pollination without requiring manual intervention. The CMS trait causes automatic pollen abortion in the female parent, ensuring that only cross-pollinated seeds are produced. This self-service mechanism eliminates the need for labor-intensive detasseling operations and simplifies hybrid seed production while maintaining high efficiency.
Data Source
AI summary
A novel maize variety designated X11T099 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X11T099 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X11T099 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X11T099, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X11T099 are provided. Methods for producing maize varieties derived from maize variety X11T099 and methods of using maize variety X11T099 are disclosed.