Jalapeno Pepper Hybrid XHP16841 Breeding via Molecular Markers

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Solution Overview

Problem

Current pepper breeding methods face challenges in identifying and developing superior pepper varieties with desirable traits such as disease resistance, nutritional quality, and environmental stress tolerance, due to the complexity of inheritance and the masking of true genotypic values by confounding traits and environmental factors.

Innovation Solution

The development of the jalapeno hybrid pepper XHP16841, which can be used in crossing and breeding programs to introduce desirable traits like herbicide tolerance, insect resistance, and enhanced nutritional quality through methods such as backcrossing, mutation breeding, and genetic transformation, utilizing techniques like CRISPR and TALENs for targeted genome editing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If traditional breeding methods are used to identify superior pepper varieties, then breeding process is simplified, but true genotypic values are masked by confounding traits and environmental factors

Engineering Contradiction:
Improveidentification accuracy of superior traitsVSAvoidbreeding method complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

Molecular markers serve as intermediaries between visible phenotypic traits and underlying genotypic values. These markers enable direct detection of desirable genetic traits (disease resistance, nutritional quality, stress tolerance) without being confounded by environmental factors or other traits, thus resolving the measurement precision problem while maintaining breeding process simplicity

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces traditional mechanical/visual selection methods with molecular-level detection techniques. Instead of observing phenotypic expressions that are masked by environmental factors, breeders use DNA-based molecular markers to directly identify genotypic values, achieving precise trait identification without the limitations of traditional approaches

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If complex breeding techniques like backcrossing and genetic transformation are employed, then desirable traits such as disease resistance and nutritional quality can be introduced, but breeding process complexity increases

Engineering Contradiction:
Improvetrait diversityVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

Molecular markers are developed and validated in advance for specific desirable traits (disease resistance, nutritional quality, stress tolerance). This preliminary action enables breeders to directly select for these traits using simple marker-assisted selection, avoiding the need for complex multi-generation backcrossing or genetic transformation procedures while still achieving the desired trait diversity

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent changes the selection parameter from phenotypic observation to genotypic detection. By using molecular markers that detect specific DNA sequences associated with desirable traits, the breeding process achieves high adaptability and trait diversity through simple selection based on marker presence, rather than complex breeding techniques

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11324190B2Jalapeno pepper hybrid XHP16841
Publication Date: 2022.05.10 SAKATA SEED AMERICA INC
  • US11324190B2 patent drawing

AI summary

One embodiment relates to seed and plants of pepper hybrid XHP16841. Another embodiment relates to the plants, seeds and tissue cultures of pepper hybrid XHP16841, and to methods for producing a pepper plant produced by crossing such plants with themselves, with another pepper plant, such as a plant of another genotype, or with vegetatively propagating said plant. Another embodiment further relates to seeds and plants produced by such crossing. Further embodiments relate to parts of such plants, including the fruit and gametes of such plants.