Anaerobic Fungal Xylanase Stabilization with Polyol
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Solution Overview
Problem
Xylanases from anaerobic fungi are prone to inactivation due to environmental factors, particularly high temperatures, leading to reduced stability and efficiency in applications such as food, animal feed, and papermaking, where their heat-resistant properties are not fully utilized.
Innovation Solution
A xylanase composition comprising a polyol, such as glycerol, sorbitol, or sucrose, with a content of at least 40 wt % based on the total weight, is used to stabilize xylanases from anaerobic fungi, enhancing their resistance to temperature-related degradation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If xylanase from anaerobic fungi is used to achieve high enzyme activity and heat resistance, then the enzyme becomes more widely applicable, but the enzyme is prone to inactivation due to environmental factors such as high temperature during storage and transport
Solution Approach 1:
The patent uses polyols (glycerol, sorbitol, or sucrose) as intermediary substances to protect xylanase from temperature-induced inactivation. These polyols act as mediators between the enzyme and the harsh environmental conditions, forming a protective environment that prevents direct damage from high temperature and other stressors, thereby maintaining enzyme stability during storage and transport.
Solution Approach 2:
The patent changes the chemical composition parameters of the storage environment by incorporating specific polyols at optimized concentrations. This parameter change transforms the storage conditions from hostile (prone to enzyme inactivation) to protective, allowing the xylanase to maintain its activity under varying temperature conditions without requiring complex equipment or procedures.
2Duration of action of stationary object
If conventional formulations with polyols, salts and antibiotics are used to stabilize xylanase, then some stabilizing effect is achieved, but the stabilizing duration and temperature are quite limited with activity dropping to about 80% after 42 hours under 48°C
Solution Approach 1:
The patent optimizes the concentration parameters of polyols in the formulation, using at least 40 wt% polyol content, which significantly extends the stabilizing duration and temperature range compared to conventional formulations. This parameter optimization allows the enzyme to maintain high activity levels for much longer periods and under higher temperature conditions.
Solution Approach 2:
The patent creates a composite stabilization system combining xylanase with specific polyols (glycerol, sorbitol, or sucrose) in optimized ratios. This composite formulation works synergistically to provide enhanced protection against inactivation, extending both the duration and temperature range of enzyme stability beyond what conventional single-component formulations can achieve.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The xylanase composition significantly extends the storage period and maintains enzyme activity under high temperatures, with residual activity ranging from 59.6% to 97% after storage at 50°C for several weeks, compared to complete inactivation without stabilization.
Implementation Method 1
enhancing their resistance to temperature-related degradation
Implementation Method 2
a xylanase composition comprising a polyol, such as glycerol, sorbitol, or sucrose, with a content of at least 40 wt % based on the total weight, is used to stabilize xylanases from anaerobic fungi
Data Source
AI summary
A xylanase composition and a method for manufacturing the xylanase composition are provided, wherein the xylanase composition comprises a xylanase and a stabilizer, and the xylanase is from an anaerobic fungus, the stabilizer comprises a polyol, and the content of the polyol is at least 40 wt %, based on the total weight of the xylanase composition.