Xylanase Polypeptides Enhance Lignocellulose Hydrolysis

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Solution Overview

Problem

There is a need to improve cellulolytic enzyme compositions for more efficient degradation of lignocellulose by supplementing with additional enzymes to enhance the conversion of lignocellulosic feedstocks into ethanol, as existing technologies face challenges in achieving cost-effective and efficient enzyme solutions.

Innovation Solution

Development of polypeptides with xylanase activity, specifically isolated polypeptides having at least 65% sequence identity to the mature polypeptide of SEQ ID NO: 2, or variants thereof, which are used in enzyme compositions to degrade or convert cellulosic and xylan-containing materials, along with methods for their production and use in fermentation processes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If existing cellulolytic enzyme compositions are used, then the degradation of lignocellulose can proceed, but the efficiency and cost-effectiveness are insufficient

Engineering Contradiction:
Improvedegradation efficiencyVSAvoidcost-effectiveness
Core Design Contradiction:
ProductivityVSEase of manufacture

Solution Approach 1:

The patent applies parameter changes by modifying the enzyme composition through supplementation with specific additional enzymes (xylanases, mannanases, pectinases, etc.) to optimize the degradation process. This resolves the contradiction by improving productivity through enhanced enzymatic activity while maintaining cost-effectiveness by targeting specific rate-limiting steps in lignocellulose degradation.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite enzyme system by combining cellulolytic enzymes with hemicellulolytic enzymes (xylanases, mannanases, pectinases) and other auxiliary enzymes. This composite approach improves degradation efficiency by addressing the complex structure of lignocellulose multiple components simultaneously, while the synergistic effects reduce overall costs compared to using larger amounts of单一 enzymes.

Inventive Principle:
Principle #40Composite materials

2Productivity

If additional enzymes are supplemented to improve efficiency, then the conversion efficiency increases, but the complexity of enzyme composition increases

Engineering Contradiction:
Improveconversion efficiencyVSAvoidenzyme composition complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent employs multi-functional enzyme compositions where a single supplemented enzyme preparation can perform multiple functions (e.g., xylanases that simultaneously address hemicellulose degradation and improve cellulose accessibility). This resolves the contradiction by achieving high conversion efficiency through versatile enzymes rather than requiring complex mixtures of many specialized enzymes.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The patent applies preliminary action by pre-treating the lignocellulose material or pre-combining enzymes in optimized formulations before the main degradation process. This allows the additional enzymes to prepare the substrate (e.g., removing hemicellulose barriers) in advance, making the subsequent cellulose degradation more efficient without requiring continuous addition of multiple enzymes during the process.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The use of these polypeptides in enzyme compositions significantly enhances the hydrolysis of lignocellulosic materials, improving the efficiency of converting biomass into ethanol, thereby addressing the inefficiencies and cost challenges of existing enzyme solutions.

Implementation Method 1

Xylanases (e.g., endo-1,4-beta-xylanase, EC 3.2.1.8) hydrolyze internal β-1,4-xylosidic linkages in xylan to produce smaller molecular weight xylose and xylo-oligomers

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

Endoglucanases digest the cellulose polymer at random locations, opening it to attack by cellobiohydrolases

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 3

Cellobiohydrolases sequentially release molecules of cellobiose from the ends of the cellulose polymer

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 4

Beta-glucosidases hydrolyze cellobiose to glucose

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 5

the glucose and xylose can be fermented by yeast into ethanol

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentUS10202592B2Polypeptides having xylanase activity and polynucleotides encoding same
Publication Date: 2019.02.12 NOVOZYMES AS
  • US10202592B2 patent drawing
  • US10202592B2 patent drawing
  • US10202592B2 patent drawing

AI summary

The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.