Yardlong Bean KASP Markers for Early Anthocyanin Trait Selection

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Solution Overview

Problem

Traditional breeding for high anthocyanin yardlong beans is time-consuming, labor-intensive, and susceptible to environmental interference with low accuracy, necessitating the development of efficient molecular markers for assisted selection.

Innovation Solution

Development of kompetitive allele-specific polymerase chain reaction (KASP) markers based on single-nucleotide polymorphism (SNP) loci, specifically at position 2,361,292 bp on chromosome 5 of the yardlong bean genome, using primers F1, F2, and R for accurate genotyping and selection of yardlong beans with high anthocyanin content.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If traditional single-plant selection based on anthocyanin content is used, then breeding can proceed with simple methods, but the process becomes time-consuming and labor-intensive with low accuracy

Engineering Contradiction:

Improveanthocyanin content detection accuracyVSAvoidbreeding time

Core Design Contradiction:

Measurement precisionVSLoss of time

Solution Approach 1:

The patent develops KASP molecular markers based on SNP loci that are significantly related to anthocyanin content before the breeding selection process. These markers enable early identification of high anthocyanin content in breeding materials at the seedling stage, eliminating the need to wait for fruit development and manual anthocyanin content measurement, thus dramatically reducing breeding time while maintaining high detection accuracy

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent replaces the traditional mechanical/visual assessment of anthocyanin content (which requires harvesting, weighing, and spectrophotometric analysis) with a molecular biology-based KASP marker system. This substitution uses PCR amplification and fluorescent detection to identify genetic markers associated with high anthocyanin content, achieving both high precision and time efficiency

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Measurement precision

If traditional single-plant selection is used, then the method is simple to operate, but it is susceptible to environmental interference with low accuracy

Engineering Contradiction:

Improveanthocyanin content detection accuracyVSAvoidbreeding operation complexity

Core Design Contradiction:

Measurement precisionVSEase of operation

Solution Approach 1:

The patent introduces KASP molecular markers as an intermediary between the target trait (anthocyanin content) and the selection process. These markers serve as genetic proxies that reflect the desired trait without being directly affected by environmental factors. The markers are detected through standardized PCR and fluorescent reading protocols, providing objective and accurate results that are not susceptible to environmental interference while maintaining operational simplicity

Inventive Principle:

Principle #24Intermediary (Mediator)

3Productivity

If KASP markers based on SNP loci are developed, then rapid and accurate selection is achieved, but the device and method complexity increases

Engineering Contradiction:

Improvebreeding efficiencyVSAvoidmolecular marker system complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The patent utilizes natural SNP (single-nucleotide polymorphism) parameter variations in the genome that are significantly associated with anthocyanin content. By designing KASP markers that detect these existing natural genetic variations, the system achieves high breeding efficiency without requiring complex artificial gene construction or modification. The approach leverages naturally occurring genetic diversity, simplifying the overall system while maintaining high productivity

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The KASP markers enable rapid and accurate identification of yardlong beans with high anthocyanin content, reducing breeding workload and accelerating the breeding process while maintaining high accuracy.

Implementation Method 1

kompetitive allele-specific polymerase chain reaction (KASP) markers based on a single-nucleotide polymorphism (SNP) locus significantly related to yardlong bean anthocyanin content

Methodology Applied

Scientific EffectPolymerase chain reaction (PCR):

Data Source

PatentUS12630888B2Single-nucleotide polymorphism locus significantly related to yardlong bean anthocyanin content, kompetitive allele-specific polymerase chain reaction markers and application thereof

Publication Date: 2026.05.19 JIANGSU ACAD OF AGRI SCI

AI summary

An SNP marker significantly related with yardlong bean anthocyanin content is provided in the present disclosure. The SNP marker significantly related with yardlong bean anthocyanin content is located at the position of 2,361,292 bp on chromosome 5 of yardlong bean genome v1.2, with a base A to G substitution, and the nucleotide sequence thereof is shown in SEQ ID NO. 1. KASP marker based on the SNP marker significantly related with the yardlong bean anthocyanin content and application thereof are also provided in the present disclosure. The KASP marker and the SNP marker significantly related with yardlong bean anthocyanin content are applied for molecular marker-assisted selection breeding of yardlong bean anthocyanin traits.