Yeast-Bacteriophage Dry Mixture for Storage Stability
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Solution Overview
Problem
Current methods for drying bacteriophages with yeasts face challenges such as low viability, instability under acidic conditions, and sensitivity to UV radiation, making it difficult to maintain the effectiveness of bacteriophages in treating bacterial infections and biocontrol applications.
Innovation Solution
A method of manufacturing a dry mixture of yeasts and/or yeast derivatives with bacteriophages, where the mixture is formed by suspending the yeast and bacteriophages together and then dried, using techniques like freeze-drying, spray-drying, or fluidized air bed drying, with the addition of drying excipients to enhance stability and viability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If bacteriophages are dried using conventional methods, then handling and storage stability are improved, but viability and effectiveness are reduced
Solution Approach 1:
The patent uses yeast cells as an intermediary carrier to protect bacteriophages during the drying process. The yeast cells serve as a protective matrix that maintains bacteriophage stability while enabling effective drying, thus resolving the contradiction between storage stability and viability.
Solution Approach 2:
The invention creates a composite structure where bacteriophages are associated with yeast cells in a dried mixture. This composite material approach allows the combination of the protective properties of yeast with the drying capability, maintaining bacteriophage effectiveness while achieving stable dry form storage.
2Ease of manufacture
If bacteriophages are exposed to acidic conditions during drying, then drying process is simplified, but bacteriophage stability deteriorates
Solution Approach 1:
Yeast cells act as a protective intermediary that shields bacteriophages from acidic conditions during the drying process. The yeast matrix buffers the acidic environment, allowing simplified drying procedures while maintaining bacteriophage stability.
3Ease of manufacture
If bacteriophages are dried without protective measures, then manufacturing cost is reduced, but sensitivity to UV radiation and environmental stress increases
Solution Approach 1:
The yeast cells serve as a protective intermediary that shields bacteriophages from harmful UV radiation and environmental stress during drying. This natural protective matrix reduces the need for additional expensive protective measures while maintaining bacteriophage effectiveness.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces stable formulations that protect bacteriophages, allowing them to maintain activity in harsh environments, such as the gastrointestinal tract, and retain their beneficial properties, improving handling and storage stability while reducing costs and handling errors.
Implementation Method 1
a method of manufacturing of a dry mixture of at least one yeast and/or yeast derivative and at least one bacteriophage... by the mixing of at least one yeast and/or at least one yeast derivative and at least one bacteriophage in suspension and the drying of this mixture
Implementation Method 2
using techniques like freeze-drying, spray-drying, or fluidized air bed drying
Implementation Method 3
using techniques like freeze-drying, spray-drying, or fluidized air bed drying
Implementation Method 4
using techniques like freeze-drying, spray-drying, or fluidized air bed drying
Data Source
AI summary
A method of manufacturing of a dry mixture of at least one yeast and/or yeast derivative and at least one bacteriophage, where the mixture being in the form of solid entities, and each solid entity is composed of at least one yeast and/or at least one yeast derivative and at least one bacteriophage and possibly at least one drying excipient, where the method is done by the mixing of at least one yeast and/or yeast derivative and at least one bacteriophage in suspension and the drying of this mixture. Also, a dry mixture of at least one yeast and/or yeast derivative and at least one bacteriophage, which is in the form of solid entities, where each solid entity is composed of at least one yeast and/or at least one yeast derivative and at least one bacteriophage and possibly at least one drying excipient and uses thereof.