Yeast Beta-Glucan Mannan Extraction Enzymatic Autolysis
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Solution Overview
Problem
Current methods for extracting β-glucan/mannan from yeast cells often require high concentrations of alkali or acid and high temperatures, leading to inefficient recovery and potential damage to the biologically useful compounds.
Innovation Solution
A method involving autolysis of yeast cells at 50°C to 65°C, followed by treatment with a high pH protease and then amylase or lipase at pH 4 to 6, to separate and enrich β-glucan and mannan components, avoiding harsh conditions and improving recovery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If high concentrations of alkali or acid and high temperatures are used for extraction, then extraction efficiency is improved, but biological activity of glucans and mannans deteriorates
Solution Approach 1:
The patent changes the extraction parameters from harsh conditions (high concentration alkali/acid and high temperature) to mild conditions (pH 2-3, temperature 20-40°C). This parameter change allows effective extraction while preserving the biological activity of the glucans and mannans, resolving the contradiction between extraction efficiency and biological activity preservation.
Solution Approach 2:
The patent replaces the mechanical/chemical extraction system (using strong alkali or acid) with an enzymatic extraction system using proteases and glucanases. This substitution enables extraction under mild conditions that preserve biological activity while maintaining effective extraction efficiency.
2Ease of manufacture
If high concentrations of alkali or acid are used for extraction, then cell wall material is broken down, but purification quality deteriorates
Solution Approach 1:
The patent replaces harsh chemical breakdown methods with enzymatic degradation using proteases and glucanases. This substitution achieves effective cell wall material breakdown while maintaining high purification quality, as the enzymes specifically target cell wall components without damaging the desired glucan and mannan products.
Solution Approach 2:
The patent introduces enzymes (proteases and glucanases) as intermediaries to mediate the breakdown of cell wall material. These enzymes act as selective agents that degrade cell wall components while preserving the integrity and purity of the extracted glucans and mannans, resolving the contradiction between breakdown efficiency and purification quality.
3Quantity of substance
If multiple extraction steps are repeated, then recovery yield is improved, but processing time and complexity increase
Solution Approach 1:
The patent employs continuous enzymatic treatment where proteases and glucanases are applied in sequence without requiring multiple separate extraction cycles. This continuous action achieves high recovery yields while minimizing processing time, as the enzymes work continuously on the cell wall material under optimized conditions rather than requiring repeated batch extractions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively produces enriched β-glucan and mannan preparations with improved yield and biological utility, suitable for various applications including food, pharmaceuticals, and animal feeds, without the need for high alkali or acid use.
Implementation Method 1
autolyzing the yeast cells at a temperature of 50 °C to 65°C to release yeast cell walls
Implementation Method 2
incubating the yeast cell walls with an exogenous protease at a pH of 9 to 10
Implementation Method 3
incubating the protease-treated cell walls of step (b) with an enzyme comprising at least one of an amylase, lipase or a combination thereof at a pH of 4 to 6
Implementation Method 4
separating the enzyme-treated cell walls of step (c) into a glucan-enriched component and a mannan-enriched component
Data Source
AI summary
Disclosed are methods for producing yeast beta-glucan and mannan preparations. The methods employ an autolysis process, followed by an enzymatic treatment using a high-PH protease or a high-PH protease followed by a treatment with an enzyme comprising at least one of an amylase, lipase and a combination thereof .