Yeast Cell Wall Composition via Enzymatic Protein Reduction
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Solution Overview
Problem
Existing processes for producing yeast cell wall compositions from Saccharomyces cerevisiae yield products with variable compositions due to different production methods and yeast strains, lacking a standardized approach to utilize exhausted yeast biomass effectively.
Innovation Solution
A process involving dispersing exhausted yeast in an aqueous medium, treating with a proteolytic enzyme at specific temperature and pH conditions, and centrifugation steps to achieve a composition with controlled protein and polysaccharide content, specifically targeting a yeast cell wall composition with 20-30% protein and 20-24% glucans, suitable for animal feed and human consumption.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If existing processes for producing yeast cell wall compositions from Saccharomyces cerevisiae are used, then products can be obtained, but the composition varies due to different production methods and yeast strains
Solution Approach 1:
The patent applies parameter changes by establishing specific controlled conditions for the enzymatic treatment process, including pH range (7.5-9.0), temperature (50-70°C), treatment time (6-10 hours), and enzyme dosage. These standardized parameters ensure consistent protein reduction and composition across different production batches, directly resolving the contradiction between composition consistency and process standardization
Solution Approach 2:
The patent employs preliminary action by first dispersing exhausted yeast in an aqueous medium to create a uniform suspension before enzymatic treatment. This preliminary step ensures consistent substrate availability and enzyme distribution, which establishes a standardized foundation for subsequent processing steps and achieves reproducible composition results
2Loss of substance
If exhausted yeast biomass is utilized, then resource efficiency improves, but protein content must be reduced to achieve desired composition
Solution Approach 1:
The patent applies the extraction principle by selectively removing protein components from exhausted yeast biomass through controlled enzymatic hydrolysis. The proteolytic enzyme specifically targets and breaks down protein structures, allowing selective extraction of excess protein while preserving the desired polysaccharide components (β-glucans and mannans), thus achieving both biomass utilization and protein content control
Solution Approach 2:
The patent uses a proteolytic enzyme as an intermediary agent to mediate the breakdown of proteins in exhausted yeast. This biological catalyst enables selective protein degradation under controlled conditions, facilitating the transformation of high-protein biomass into the desired low-protein cell wall composition with 20-30% protein content while maintaining resource efficiency
3Manufacturing precision
If proteolytic enzyme treatment is applied to reduce protein content, then composition quality improves, but processing time increases
Solution Approach 1:
The patent optimizes the balance between protein reduction quality and processing time by establishing specific parameter ranges: pH 7.5-9.0, temperature 50-70°C, and treatment time 6-10 hours. These optimized parameters maximize enzymatic activity and protein breakdown efficiency while minimizing unnecessary processing time, achieving both composition quality and time efficiency
Solution Approach 2:
The patent applies preliminary action by preparing the yeast suspension in advance with appropriate pH adjustment and temperature conditioning before enzyme addition. This preliminary preparation ensures optimal conditions for enzymatic activity from the start, reducing the overall treatment time required to achieve the desired protein content reduction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process standardizes the production of yeast cell walls with consistent glucan and mannan content, enhancing their suitability as a food supplement and feed additive, while efficiently utilizing exhausted yeast biomass, thereby improving product quality and value.
Implementation Method 1
treating said first suspension having a dry weight of between 5% and 12% with a proteolytic enzyme at a temperature comprised between 50°C and 70°C, for 6 to 10 hours
Implementation Method 2
c) centrifuging said first suspension obtaining a first pellet and a supernatant
Data Source
AI summary
Process for the production of a composition substantially consisting of yeast cell walls, which comprises steps of dispersing exhausted yeast cells in an aqueous medium to obtain a first suspension of yeast cells; treating the suspension with a proteolytic enzyme; centrifuging the suspension obtaining a first pellet and a supernatant; dispersing the first pellet in an aqueous medium, obtaining a second suspension of yeast cells; centrifuging the second suspension obtaining a second pellet, which constitutes the above-mentioned composition substantially consisting of yeast cell walls.