Yeast Cell Composition for Wine Oxidation Stabilization

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current methods for stabilizing aqueous food liquids, particularly wines, against oxidation during storage are ineffective, leading to undesirable changes in physicochemical and organoleptic properties, such as browning and loss of varietal aromas, due to the limitations of existing antioxidants and the difficulty in completely excluding oxygen contact.

Innovation Solution

A composition comprising non-viable yeast cells capable of rapidly consuming oxygen and inactivated yeast cells enriched in glutathione is used to stabilize aqueous food liquids, reducing oxidation reactions and maintaining the organoleptic qualities of the liquid by forming colorless complexes with quinones.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical antioxidants such as sulphite are added to prevent oxidation, then oxidation resistance is improved, but organoleptic quality deteriorates due to sulfur odor and regulatory limitations

Engineering Contradiction:
Improveoxidation resistanceVSAvoidorganoleptic quality
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses ascorbic acid as an intermediary substance that preferentially reacts with oxygen and quinones, protecting the wine's aromatic compounds without introducing harmful odors. The ascorbic acid acts as a mediator between oxygen and the sensitive aromatic molecules, sacrificing itself to preserve the wine's organoleptic quality while providing effective oxidation protection

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent employs ascorbic acid as a consumable antioxidant that gets depleted over time, replacing the need for persistent chemical additives like sulphite. This disposable approach allows for effective oxidation protection during the critical storage period without long-term organoleptic degradation, and the depleted antioxidant can be easily replaced

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

2Reliability

If polyphenols are eliminated using fining agents to prevent browning, then oxidation resistance is improved, but flavor and aroma are altered negatively

Engineering Contradiction:
Improveoxidation resistanceVSAvoidflavor and aroma
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent selectively extracts or removes quinones from the wine matrix through reaction with ascorbic acid, while leaving the beneficial polyphenols and aromatic compounds intact. This selective extraction approach prevents browning without eliminating the compounds responsible for flavor and aroma, resolving the contradiction between oxidation resistance and organoleptic quality

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent applies oxidation protection locally to the specific molecules that cause browning (quinones) rather than globally removing all polyphenols. The ascorbic acid targets quinones preferentially, providing localized oxidation protection at the molecular level where it is most needed, while preserving the overall polyphenol content and its contribution to flavor and aroma

Inventive Principle:
Principle #3Local quality

3Reliability

If oxygen contact is completely excluded during storage, then oxidation resistance is improved, but packaging complexity and cost increase

Engineering Contradiction:
Improveoxidation resistanceVSAvoidpackaging complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent enables the wine to protect itself from oxidation through the addition of ascorbic acid, which actively consumes oxygen and quinones during storage. This self-service approach eliminates the need for complex oxygen-exclusion packaging systems, as the wine autonomously neutralizes oxidizing agents through the chemical action of the antioxidant

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent converts the harmful presence of oxygen and quinones into a beneficial process by using ascorbic acid to trigger selective reactions that remove the harmful oxidizing agents. The oxygen that would normally cause oxidation is instead consumed in a controlled chemical reaction with ascorbic acid, transforming a harmful factor into a controlled protective mechanism

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The combination effectively prevents oxidation-induced browning and aroma loss in wines, maintaining the liquid's original color and flavor profile, thus extending shelf life and preserving the wine's quality.

Implementation Method 1

non-viable yeast cells capable of rapidly consuming oxygen

Methodology Applied
Scientific EffectCellular respiration: Aerobic Digestion

Implementation Method 2

inactivated yeast cells produced to be rich in glutathione... forming colorless complexes with quinones

Methodology Applied
Scientific EffectComplexation: Chemical Bonding

Implementation Method 3

oxygen can diffuse through a cork or come from the gaseous headspace under the cork after bottling

Methodology Applied
Scientific EffectDiffusion: Diffusion

Implementation Method 4

the browning of white wines is attributed to oxidative polymerization reactions of certain polyphenols

Methodology Applied
Scientific EffectOxidative polymerization: Oxidation

Data Source

PatentEP2247205B8Composition for stabilising a dietary aqueous liquid sensitive to oxidation
Publication Date: 2013.03.27 INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

AI summary

The invention relates to a composition for protecting a dietary liquid, which contains substances sensitive to oxidation during the shelf-life thereof, from oxidation. Said composition includes a combination of two types of yeast cells: (i) non-viable yeast cells capable of rapidly consuming oxygen; and (ii) glutathion-enriched inactivated yeast cells.