Yeast Fermentation Control for Higher SOD Biosynthesis Productivity
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for producing superoxide dismutase (SOD) by microbial biosynthesis face low productivity, characterized by low yeast content, low SOD content in yeast, and lengthy biosynthesis times, leading to high production costs and difficulties in obtaining high-purity SOD.
Innovation Solution
A method involving high yeast inoculation, controlled conditions such as air flow rate, stirring speed, and pH, along with continuous addition of carbon and nitrogen sources, and copper and zinc salts during the biosynthesis process, to enhance yeast content and SOD activity in the fermentation medium.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microbial biosynthesis methods are used to produce superoxide dismutase, then the production process is simple, but the productivity is low due to low yeast content, low SOD content in yeast, and lengthy biosynthesis times
Solution Approach 1:
The patent applies preliminary action by pre-culturing yeast cells before the main biosynthesis phase. The yeast is first cultured in a preliminary fermentation stage to establish a high cell density and metabolic activity baseline, then transferred to the main fermentation tank for SOD production. This preliminary preparation ensures that when SOD biosynthesis begins, the yeast cells are already in an optimal state for rapid enzyme production, significantly reducing the overall biosynthesis time and improving productivity.
Solution Approach 2:
The patent implements continuity of useful action through a multi-stage fermentation process where yeast cultivation and SOD biosynthesis occur in continuous succession without interruption. The preliminary culture phase is directly followed by the main biosynthesis phase, with nutrients and conditions optimized to maintain continuous metabolic activity. This continuous process eliminates idle time between stages and ensures that yeast cells continuously produce SOD throughout the fermentation period, maximizing productivity.
2Productivity
If conventional biosynthesis methods are used, then the process is straightforward, but high production costs result from low yeast content and low SOD content in yeast
Solution Approach 1:
The patent applies parameter changes by systematically optimizing multiple fermentation parameters including temperature, pH, carbon source concentration, nitrogen source composition, and aeration rates. These parameters are adjusted specifically to maximize yeast cell growth and SOD enzyme production. By controlling these parameters, the patent achieves high yeast content (increasing the substrate for SOD production) and high SOD content in the yeast cells, thereby improving productivity and reducing production costs.
Solution Approach 2:
The patent uses composite materials in the form of enriched culture media containing multiple nutrients designed to support both yeast growth and SOD synthesis. The media include composite carbon sources, nitrogen sources, vitamins, and mineral salts that work synergistically to enhance yeast cellular metabolism and SOD biosynthesis. This composite approach ensures that yeast cells have all necessary building blocks to produce high quantities of SOD enzyme, increasing both yeast content and SOD content simultaneously.
3Manufacturing precision
If conventional methods are used, then the production process is simple, but obtaining high-purity SOD becomes difficult due to low SOD content in yeast
Solution Approach 1:
The patent implements feedback control by monitoring SOD activity and yeast growth parameters throughout the fermentation process. Sampling and analysis are performed at multiple time points to assess SOD content and purity, with adjustments made to nutrient addition rates, aeration, and temperature based on these measurements. This feedback mechanism ensures that the fermentation conditions remain optimal for producing high-purity SOD, allowing the process to achieve both high productivity and high manufacturing precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases yeast content and SOD biological activity, shortening biosynthesis time and enhancing productivity, enabling high-purity SOD production suitable for industrial-scale applications.
Implementation Method 1
The microbial biosynthesis method is the subject of the presently disclosed biosynthesis method. The microorganisms used for the production of superoxide dismutase (SOD) by microbial biosynthesis are mainly yeast and bacteria
Implementation Method 2
Production of superoxide dismutase (SOD) by genetic engineering method has only been studied in recent years. The microbial biosynthesis method is the subject of the presently disclosed biosynthesis method
Implementation Method 3
The main function of superoxide dismutase (SOD) is to catalyze the dismutation of superoxide anion free radicals into hydrogen peroxide and oxygen
Implementation Method 4
It is an antioxidant metalloenzyme that can catalyze the dismutation of superoxide anion free radicals to generate oxygen and hydrogen peroxide
Implementation Method 5
Superoxide dismutase (SOD) can remove and reduce excessive free radicals in the human body and delay aging
Data Source
AI summary
The present invention discloses a method for increasing the productivity of biosynthetic superoxide dismutase (SOD). In the initial stage, a high yeast inoculation amount is used, combined with the addition of carbon sources, nitrogen sources and nutrients required for biosynthesis, and by increasing the dissolved oxygen value in the culture medium, using the optimal temperature and pH value, so that the yeast content in the culture medium increases rapidly in the middle stage of biosynthesis. After the middle stage of biosynthesis, the method of continuously adding carbon sources and nitrogen sources is adopted to continuously provide the biosynthetic superoxide dismutase (SOD) with the energy substances required for yeast biosynthesis. Combined with increasing the dissolved oxygen value in the culture medium and continuously adding copper and zinc salts to the fermentation tank, the yeast is stimulated to quickly synthesize copper and zinc superoxide dismutase (SOD) in the body. Through the above method, the yeast content and the superoxide dismutase (SOD) content in the yeast are increased, and the overall biosynthesis time is shortened, ultimately increasing the productivity of superoxide dismutase (SOD) biosynthesis.