Yeast Strain for High-Yield Insecticidal Peptide Production
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Solution Overview
Problem
The commercialization of insecticidal peptides is hindered by their delicacy, toxicity levels, production costs, rapid degradation, and loss of toxicity when expressed in genetically modified organisms or consumed by insects, with few peptides successfully reaching the market due to these challenges, particularly concerning the rise of Bacillus thuringiensis-resistant insects.
Innovation Solution
The development of special expression cassettes and systems for plants to properly fold and express insecticidal peptides, modification of peptides for increased production yields and cost-effectiveness, and combination of different peptide classes to operate synergistically and maintain toxicity, including the use of modified yeast cells for heterologous polypeptide production and sugar alcohol-based fermentation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If insecticidal peptides are expressed in genetically modified organisms, then production yield is improved, but toxicity is lost due to improper folding
Solution Approach 1:
The patent introduces a disulfide bond stabilizing peptide as an intermediary component that facilitates proper folding of insecticidal peptides in GM organisms. This stabilizing peptide acts as a molecular chaperone that ensures correct disulfide bond formation, thereby maintaining toxicity while enabling high-yield production in transgenic systems.
Solution Approach 2:
The patent creates a composite peptide structure by fusing the insecticidal peptide with a disulfide bond stabilizing peptide. This composite structure ensures proper folding and stability in GM organisms, allowing both high production yield and retained toxicity to be achieved simultaneously.
2Ease of manufacture
If conventional production methods are used, then production cost is reduced, but peptide degradation occurs rapidly
Solution Approach 1:
The patent employs a cost-effective disulfide bond stabilizing peptide sequence that can be produced inexpensively and integrated into the insecticidal peptide. This stabilizing component significantly extends peptide half-life and stability without adding substantial production cost, effectively addressing both affordability and durability requirements.
3Object-affected harmful factors
If Bt proteins are used for crop protection, then insect control is improved, but insect resistance develops
Solution Approach 1:
The patent modifies the peptide sequence by incorporating a disulfide bond stabilizing peptide domain, which changes the structural and functional parameters of the insecticidal peptide. This modification creates a novel peptide with enhanced stability and potentially different modes of action, making it effective against Bt-resistant insects while maintaining crop protection capabilities.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the production of properly folded, high-yield, and cost-effective insecticidal peptides that retain toxicity, effectively controlling both susceptible and Bacillus thuringiensis-resistant insects, thereby addressing the global threat of insect resistance and ensuring crop protection.
Implementation Method 1
High gene expression yeast strain
Implementation Method 2
sugar alcohol-based fermentation
Implementation Method 3
Cysteine-Rich Bioactive Peptides (CRBPs) are peptides, polypeptides, and/or proteins that possess cysteine residues capable of forming disulfide bonds; these disulfide bonds create a scaffolding motif
Data Source
AI summary
New insecticidal proteins, nucleotides, peptides, their expression in plants, methods of producing the peptides, new processes, production techniques, new peptides, new formulations, new organisms, and a process which increases the insecticidal peptide production yield from yeast expression systems. The present invention is also related to novel cell culture methods and conditions that can be used to express heterologous polypeptides, along with new transgenic yeast strains.