Yeast Mash Exosome Isolation for Cancer Suppression and Tissue Elasticity
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Solution Overview
Problem
Existing technologies have not effectively utilized yeast-derived exosomes for their potential health benefits, particularly in preventing metabolic syndrome, improving tissue elasticity, and suppressing cancer.
Innovation Solution
Exosomes are extracted from yeast mash and miso using a unique production method involving centrifugation and ultracentrifugation, and are incorporated into compositions for health maintenance, cancer suppression, and tissue elasticity improvement.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If exosomes are extracted from yeast mash or miso using conventional methods, then extraction efficiency is low, but using centrifugation and ultracentrifugation improves extraction efficiency while increasing process complexity
Solution Approach 1:
The extraction process is divided into multiple stages: initial centrifugation to remove large particles, followed by ultracentrifugation to isolate exosomes, and final purification steps. This segmentation allows each step to target specific size ranges, improving overall extraction efficiency while managing complexity through systematic breakdown of the process
Solution Approach 2:
The patent uses density gradient media as an intermediary substance during ultracentrifugation to facilitate exosome separation. This intermediary creates distinct density layers that enable precise isolation of exosomes from other cellular components, enhancing extraction efficiency without requiring overly complex equipment
2Quantity of substance
If yeast mash or miso is used as raw material, then exosome source is abundant and cost-effective, but purification difficulty increases
Solution Approach 1:
The patent systematically extracts exosomes from complex yeast mash and miso matrices through sequential separation steps. Each extraction step removes specific contaminants while preserving exosomes, gradually purifying the sample from the complex raw material without losing valuable exosome quantity
Solution Approach 2:
The purification process exploits parameter changes in density, size, and surface properties during centrifugation and filtration steps. By adjusting these parameters at each stage, the method effectively separates exosomes from the complex yeast mash and miso background, reducing purification difficulty while maintaining high exosome recovery
3Manufacturing precision
If exosomes are isolated through multiple centrifugation steps, then purity increases, but processing time increases
Solution Approach 1:
The patent performs preliminary centrifugation steps to remove large cellular debris and contaminants before the main exosome isolation step. This preliminary action prevents interference in subsequent ultracentrifugation, achieving high purity faster by addressing major contaminants early in the process
Solution Approach 2:
The method uses optimized ultracentrifugation parameters to rapidly pellet exosomes in a single high-speed step, skipping intermediate washing steps that would extend processing time. This rushed approach through the critical isolation step maintains high purity while minimizing time loss
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The extracted exosomes enhance immune activity, promote collagen and elastin production, and inhibit cancer cell proliferation, contributing to immune system activation and anti-aging effects.
Implementation Method 1
the amount of intracellular granzyme increases by addition of exosomes purified from yeast mash and moromi
Implementation Method 2
expression levels of collagen and elastin were increased
Implementation Method 3
obtained supernatant by centrifuging the yeast mash or moromi of sake at 10,000 to 20,000×g, and ultracentrifuging the supernatant at 10,000 to 210,000×g
Implementation Method 4
an immune activity of NK cells is enhanced by adding the exosomes collected from miso
Data Source
AI summary
An object of the present invention is to prepare a novel food-derived exosome composition and to provide a cancer inhibitor or an agent for preventing decrease in tissue elasticity or an agent for improving elasticity containing a novel active ingredient. As a result of intensive studies on the purification process, the inventors have found that an exosome can be extracted from yeast mash and moromi by a unique production method. The inventors have found that the exosome recovered from the yeast mash and the moromi has a cancer cell proliferation inhibition ability, an ability to promote increase in the amount of granzyme, and an ability to promote expression of collagen and elastin. Accordingly, the inventors provide a composition for treating or preventing cancer and an agent for preventing decrease in tissue elasticity or an agent for improving elasticity, containing exosomes derived from yeast mash and moromi of sake.